Investigation of the Effects of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) on Apoptosis and Cell Cycle in a Zebrafish (Danio rerio) Liver Cell Line.
Investigation of the Effects of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) on Apoptosis and Cell Cycle in a Zebrafish (Danio rerio) Liver Cell Line.
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DOI：
10.3390/ijerph121215012
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发表时间：
2015-12-09
影响因子：
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通讯作者：
Chen H
中科院分区：
文献类型：
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作者：
Cui Y;Liu W;Xie W;Yu W;Wang C;Chen H
本研究旨在探讨全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）对斑马鱼（Danio rerio）肝细胞系（ZFL）凋亡和细胞周期的影响。治疗组分为对照组、PFOA-IC50组、PFOA-IC80组、PFOS-IC50组和PFOS-IC80组。通过细胞建模和MTT测定IC50和IC80浓度。采用qPCR检测p53、Bcl-2、Bax、Caspase-3、NF-κB p65 mRNA表达水平。流式细胞术检测细胞凋亡和细胞周期，western blotting检测p53、Bcl-2、Bax、Caspase-3和NF-κB p65蛋白水平。PFOA和PFOS均能抑制斑马鱼肝细胞的生长，且PFOS的抑制率高于PFOA。4组Bcl-2表达水平均显著高于对照组，PFOA-IC80组Bcl-2表达水平显著升高。而Bax在4个治疗组的表达水平均高于对照组。PFOA和PFOS处理后细胞凋亡率均显著升高（p < 0.05）。PFOA-IC80和PFOS-IC80组细胞周期和细胞增殖均被阻断，表明PFOA-IC80和PFOS-IC50增强了ZFL细胞的凋亡。
This study aimed to explore the effects of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) on apoptosis and cell cycle in a zebrafish (Danio rerio) liver cell line (ZFL). Treatment groups included a control group, PFOA-IC50, PFOA-IC80, PFOS-IC50 and PFOS-IC80 groups. IC50 and IC80 concentrations were identified by cellular modeling and MTT assays. mRNA levels of p53, Bcl-2, Bax, Caspase-3 and NF-κB p65 were detected by qPCR. Cell apoptosis and cell cycle were detected by flow cytometry and the protein levels of p53, Bcl-2, Bax, Caspase-3 and NF-κB p65 were determined by western blotting. Both PFOA and PFOS inhibited the growth of zebrafish liver cells, and the inhibition rate of PFOS was higher than that of PFOA. Bcl-2 expression levels in the four groups were significantly higher than the control group and Bcl-2 increased significantly in the PFOA-IC80 group. However, the expression levels of Bax in the four treatment groups were higher than the control group. The percentage of cell apoptosis increased significantly with the treatment of PFOA and PFOS (p < 0.05). Cell cycle and cell proliferation were blocked in both the PFOA-IC80 and PFOS-IC80 groups, indicating that PFOA-IC80 and PFOS-IC50 enhanced apoptosis in ZFL cells.