Detection of Changes in DNA Methylation Induced by Low-Energy Ion Implantation in Arabidopsis thaliana
Detection of Changes in DNA Methylation Induced by Low-Energy Ion Implantation in Arabidopsis thaliana
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DOI：
10.1667/rr2209.1
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发表时间：
2011-03
期刊：
影响因子：
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通讯作者：
Hai-tao Yu;Jin Zhao;Jing Xu;Xiaoqu Li;Fengshou Zhang;Yugang Wang;Christopher W. Carr;Jun Zhang-Jun
中科院分区：
文献类型：
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作者：
Hai-tao Yu;Jin Zhao;Jing Xu;Xiaoqu Li;Fengshou Zhang;Yugang Wang;Christopher W. Carr;Jun Zhang-Jun
摘要本研究评估了低能N+和Ar+离子注入拟南芥种子后，拟南芥植株DNA甲基化的变化。甲基化敏感性扩增多态性（MSAP）检测显示，在1 × 1014至1 × 1016离子/cm 2的剂量下离子注入后，DNA甲基化模式发生了改变。MSAP电泳图谱的比较显示9种类型的多态性在离子注入幼苗相对于对照幼苗，其中4个代表甲基化事件，3个代表去甲基化事件，和两个甲基化状态是不确定的。低能离子注入增加了植物DNA甲基化的多样性。与此同时，离子注入后CCGG位点的总基因组DNA甲基化水平没有变化。此外，在N+离子注入，Ar+离子注入，和控制DNA中看到的多态性的比较表明，入射离子的种类影响所得的DNA甲基化模式。8个分离的片段，在植入植物中表现出不同的变化模式的测序允许其映射到一个或多个的五个拟南芥染色体上的可变区，这些片段包括蛋白质编码基因，转座子和重复DNA序列。三个片段的进一步亚硫酸氢钠测序也显示不同类型或剂量的入射离子之间的甲基化改变。甲基化的变化的可能原因进行了讨论。
Abstract This study evaluated changes in DNA methylation in Arabidopsis thaliana plants grown from seeds implanted with low-energy N+ and Ar+ ions. Methylation-sensitive amplified polymorphism (MSAP) testing revealed altered DNA methylation patterns after ion implantation at doses of 1 × 1014 to 1 × 1016 ions/cm2. Comparison of the MSAP electrophoretic profiles revealed nine types of polymorphisms in ion-implanted seedlings relative to control seedlings, among which four represented methylation events, three represented demethylation events, and the methylation status of two was uncertain. The diversity of plant DNA methylation was increased by low-energy ion implantation. At the same time, total genomic DNA methylation levels at CCGG sites were unchanged by ion implantation. Moreover, a comparison of polymorphisms seen in N+ ion-implanted, Ar+ ion-implanted, and control DNA demonstrated that the species of incident ion influenced the resulting DNA methylation pattern. Sequencing of eight isolated fragments that showed different changing patterns in implanted plants allowed their mapping onto variable regions on one or more of the five Arabidopsis chromosomes; these segments included protein-coding genes, transposon and repeat DNA sequence. A further sodium bisulfite sequencing of three fragments also displayed alterations in methylation among either different types or doses of incident ions. Possible causes for the changes in methylation are discussed.