Virulence, antimicrobial resistance and phylogenetic analysis of zoonotic walking pneumonia Mycoplasma arginini in the one-humped camel (Camelus dromedarius)
Virulence, antimicrobial resistance and phylogenetic analysis of zoonotic walking pneumonia Mycoplasma arginini in the one-humped camel (Camelus dromedarius)
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DOI：
10.1016/j.actatropica.2020.105500
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发表时间：
2020-07-01
期刊：
影响因子：
2.7
通讯作者：
Osman, Kamelia M.
中科院分区：
文献类型：
--
作者：
Abdelazeem, Walaa Mohammed;Zolnikov, Tara Rava;Osman, Kamelia M.
在科学文献中，发现了关于骆驼种属支原体病的少量信息。多种病原体均可引起肺炎，但行走性肺炎多由支原体引起，发展缓慢，症状轻微。本研究的目的是鉴定骆驼（骆驼）中的支原体，并扩展与支原体致病性有关的因子库。Escherini揭示了目前的知识差距。从单峰骆驼（C. domedarius）从苏丹进口，在开罗屠宰场屠宰。从肺炎肺中回收的210株分离株中有48株（22.9%）被记录为M。我是阿奎尼尼所有48个分离株均获得阳性PCR结果。另一方面，在明显健康的肺中未检测到该生物体的感染。溶血和硫化氢（H2S）的产生，一种以前未被鉴定为M.在100%的分离株中，Escherichini明显。48 M。在聚苯乙烯表面上形成生物膜的能力较弱。所有菌株对氟苯尼考和链霉素100%敏感，对环丙沙星100%耐药。对林可霉素、螺旋霉素、泰乐菌素、多西环素和红霉素的耐药频率不同。代表1至4类的13种不同抗生素组合是明显的，其中大环内酯红霉素是最具代表性的。还应注意的是，在21/48株分离株中，环丙沙星、多西环素、林可霉素、红霉素组合最常见。PCR未检测到毒力基因（vsp、uvrC和gapA）和喹诺酮类耐药基因（parC和gyrA）。
In the scientific literature, a small amount of information is found concerning mycoplasmosis in camel species. A variety of pathogens could be causative agents for pneumonia, but walking pneumonia is mostly caused by Mycoplasma with slow development and mild symptoms. The aim of this study was to identify mycoplasmas from camels (Camelus dromedarius) and extending the arsenal of factors implicated in pathogenicity of M. arginini to shed light on the current knowledge gap. 460 lung samples (pneumonic; n = 210 and apparently healthy; n= 250) were randomly collected from the one-humped camels (C. domedarius) that have been imported from Sudan and slaughtered at Cairo Slaughterhouse. 48 out of 210 isolates (22.9%) recovered from the pneumonic lungs were recorded as M. arginini. Positive PCR results were obtained for all 48 isolates. On the other hand, infection with the organism was not detected in the apparently healthy lungs. Hemolysis and hydrogen sulphide (H2S) production, a compound that has previously not been identified as a virulence factor in M. arginini, was evident in 100% of the isolates. The 48 M. arginini isolates were weak in their ability to form biofilm on polystyrene surfaces. All isolates were 100% susceptible to florfenicol and streptomycin and 100% resistant to ciprofloxacin. Resistance to lincomycin, spiromycin, tylosin, doxacyclin and erythromycin was observed at different frequencies. 13 different combinations of antibiotics representing one to four classes were evident with the Macrolide erythromycin being the most represented. It also should be noted that the ciprofloxacin, doxacyclin, lincomycin, erythromycin combination was the most noted in 21/48 isolates. Surprisingly, none of the virulence genes (vsp, uvrC and gapA) and quinolone resistance genes (parC and gyrA) were detected by PCR.