Emerging role for RNA-based regulation in plant immunity.
Emerging role for RNA-based regulation in plant immunity.
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DOI：
10.1111/nph.12022
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
D. Staiger;Christin Korneli;M. Lummer;Lionel Navarro
中科院分区：
文献类型：
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作者：
D. Staiger;Christin Korneli;M. Lummer;Lionel Navarro
植物病原细菌的感染引发植物基因表达的巨大变化，这被认为主要是转录重编程的结果。然而，越来越多的证据表明，植物还使用免疫应答mRNA的转录后调节作为塑造防御相关转录组的战略武器。细胞RNA结合蛋白调节免疫应答转录物的RNA稳定性、剪接或mRNA输出。特别地，抗性基因的选择性剪接缺陷的突变体表现出受损的抗病性。此外，细菌病原体的检测诱导小的非编码RNA的差异表达，包括影响宿主防御转录组的microRNA。植物病原细菌反过来又进化出效应蛋白来抑制细胞微小RNA的生物发生和/或活性。尽管RNA沉默长期以来被认为是一种抗病毒防御反应，但最近的研究结果也揭示了这一过程在抗菌防御中的主要作用。本文综述了RNA结合蛋白和小RNA介导的转录后调控在抗菌防御中的作用。我们主要集中在使用模式系统拟南芥的研究，也讨论了从其他植物中选择的例子。
Infection by phytopathogenic bacteria triggers massive changes in plant gene expression, which are thought to be mostly a result of transcriptional reprogramming. However, evidence is accumulating that plants additionally use post-transcriptional regulation of immune-responsive mRNAs as a strategic weapon to shape the defense-related transcriptome. Cellular RNA-binding proteins regulate RNA stability, splicing or mRNA export of immune-response transcripts. In particular, mutants defective in alternative splicing of resistance genes exhibit compromised disease resistance. Furthermore, detection of bacterial pathogens induces the differential expression of small non-coding RNAs including microRNAs that impact the host defense transcriptome. Phytopathogenic bacteria in turn have evolved effector proteins to inhibit biogenesis and/or activity of cellular microRNAs. Whereas RNA silencing has long been known as an antiviral defense response, recent findings also reveal a major role of this process in antibacterial defense. Here we review the function of RNA-binding proteins and small RNA-directed post-transcriptional regulation in antibacterial defense. We mainly focus on studies that used the model system Arabidopsis thaliana and also discuss selected examples from other plants.