Hydrolysis, Photolysis, and Biotoxicity Assessment of a Novel Biopesticide, Guvermectin
Hydrolysis, Photolysis, and Biotoxicity Assessment of a Novel Biopesticide, Guvermectin
复制标题
新型生物农药 Guvermectin 的水解、光解和生物毒性评估
DOI：
10.1021/acs.jafc.2c05988
复制
发表时间：
2022
影响因子：
6.1
通讯作者：
Yongquan Zheng
中科院分区：
文献类型：
--
作者：
Yuan Shi;Xiaokang An;Binbin Zhang;Xinglu Pan;Xiaohu Wu;Jun Xu;Wensheng Xiang;Fengshou Dong;Xiangjing Wang;Yongquan Zheng
阿维菌素是从链霉菌次级代谢产物中分离得到的一种生物农药。NEAU 6是一株来源于中药七叶一枝花的内生放线菌。但其环境降解行为和生物毒性尚不清楚，可能影响其合理应用。因此，在实验室中研究了在不同pH值（pH 4、pH 6、pH 7和pH 9）和有光或无光条件下古维菌素在水中的降解。结果表明，在pH 4的溶液中，阿维菌素可被降解，光照对阿维菌素的降解有促进作用，光解和水解的DT 50分别为2.95和12 d。然而，古维菌素在其他条件下相当稳定。通过UPLC-QTOF/MS鉴定了3种阿维菌素降解转化产物，并通过ECOSAR预测和体内生物学试验对斑马鱼和大型蚤进行了生物毒性评价。试验结果表明，阿维菌素及其转化产物对D. rerio和D. magnaStraus（LC50/EC50> 100 mg a.i./ L），且转化产物的毒性低于其母体。研究结果为阐明古维菌素对非靶生物的潜在风险，促进其合理使用提供了参考。
Guvermectin is a biopesticide isolated from the secondary metabolites ofStreptomycessp. NEAU6, an endogenous actinomyces of a Chinese medicine namedParis polyphylla. However, the environmental degradation behavior and biotoxicity of guvermectin are still unclear, which may affect its rational application. Therefore, the degradation of guvermectin in water at different pH values (pH 4, pH 6, pH 7, and pH 9) and with or without light was investigated in the laboratory. The results showed that guvermectin could be degraded in pH 4 solution, and the presence of light irradiation enhanced the degradation process with a DT50of 2.95 and 12 days for photolysis and hydrolysis, respectively. However, guvermectin was fairly stable in other conditions. Three products transformed from guvermectin degradation were identified by UPLC-QTOF/MS. Biotoxicity assessment was performed onDanio rerioandDaphnia magnaStraus by ECOSAR prediction and in vivo biological tests. The test data showed that guvermectin and its transformation products exhibited low toxicities toD. rerioandD. magnaStraus (LC50/EC50> 100 mg a.i./L), and the transformation products had lower toxicity than their parent substance. The results provided a reference for elucidating the potential risk of guvermectin to nontarget organisms and promoting its rational use.