Association of p63 with proliferative potential in normal and neoplastic human keratinocytes
Association of p63 with proliferative potential in normal and neoplastic human keratinocytes
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DOI：
10.1046/j.1523-1747.1999.00780.x
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发表时间：
1999-12-01
影响因子：
6.5
通讯作者：
Green, H
中科院分区：
文献类型：
--
作者：
Parsa, R;Yang, A;Green, H
P63是新近发现的P53基因家族成员，它编码多种具有反式激活、诱导死亡和显性负活性的产物。我们发现，在正常人表皮、毛囊和分层培养的表皮细胞中，P63蛋白主要局限于高增殖潜能的细胞，在终末分化的细胞中不存在。在正常人表皮和毛囊中，富含p63的基底细胞与少量或不含p63的细胞夹杂在一起，每当有p63mRNA存在时，其编码的主要是截短的、潜在的显性-阴性亚型。在鳞癌中，含有p63的细胞的数量及其分布取决于不同的不同发育程度。在分化程度较高的肿瘤中，p63仅局限于围绕终末分化中心的基底样细胞环，而在分化较低的肿瘤中，大多数细胞含有p63，其分布相对于终末分化中心是混乱的。P63是诊断间变性角质形成细胞的有价值的标志物。
p63, a recently identified member of the p53 gene family, encodes multiple products with transactivating, death-inducing, and dominant-negative activities, We show that in normal human epidermis, in hair follicles, and in stratified epidermal cultures, p63 protein is principally restricted to cells with high proliferative potential and is absent from the cells that are undergoing terminal differentiation. In normal human epidermis and in hair follicles, basal cells with abundant p63 are interspersed with cells with little or no p63, Whenever p63 mRNA is present, it encodes mainly truncated, potentially dominant-negative isotypes, In squamous cell carcinomas, the number of cells containing p63 and their distribution depends on the degree of anaplasia. In highly differentiated tumors, p63 is confined to a ring of basal-like cells surrounding, but at a distance from, centers of terminal differentiation, In less differentiated tumors, most cells contain p63 and their distribution is chaotic with respect to centers of terminal differentiation. p63 appears to be a valuable diagnostic marker for anaplastic keratinocytes.