Probing the size limit for nanomedicine penetration into Burkholderia multivorans and Pseudomonas aeruginosa biofilms
Probing the size limit for nanomedicine penetration into Burkholderia multivorans and Pseudomonas aeruginosa biofilms
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DOI：
10.1016/j.jconrel.2014.07.061
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发表时间：
2014-12-10
影响因子：
10.8
通讯作者：
Braeckmans, Kevin
中科院分区：
文献类型：
--
作者：
Forier, Katrien;Messiaen, Anne-Sophie;Braeckmans, Kevin
将抗生素封装到纳米颗粒中是消除生物膜的潜在策略。为了允许进一步优化用于生物膜根除的纳米药物，需要研究纳米颗粒尺寸对渗透到致密生物膜簇中的影响。在本研究中，使用共聚焦显微镜评估了直径范围为40至550 nm的纳米颗粒进入两种生物膜，多食伯克霍尔德氏菌LMG 18825和铜绿假单胞菌LMG 27622。通过图像分析，计算能够渗透到致密生物膜簇中的颗粒的百分比。对于两种生物膜，最佳渗透到生物膜簇中的尺寸截止位于约100-130 nm。生物膜基质的网目尺寸和簇的细菌之间的通道的尺寸是可能在排除较大颗粒中起作用的两个因素。为了B。对于多噬菌体，对于大于130 nm的颗粒，观察到进入簇的渗透率急剧下降，而对于铜绿假单胞菌，可以观察到渗透率的更逐渐的下降。铜绿假单胞菌的纳米颗粒的总渗透略低于B。基于这些结果，可以得出结论，约100 nm和更小的纳米载体是改善CF患者中慢性肺生物膜治疗的良好候选物。此外，共聚焦显微镜的方法证明这里是一个有用的工具，以评估纳米药物在生物膜集群的渗透。这些信息对于优化用于治疗生物膜感染的纳米药物制剂是重要的。(C)2014爱思唯尔有限公司版权所有。
Encapsulation of antibiotics into nanoparticles is a potential strategy to eradicate biofilms. To allow further optimization of nanomedicines for biofilm eradication, the influence of the nanoparticle size on the penetration into dense biofilm clusters needs to be investigated. In the present study, the penetration of nanoparticles with diameters ranging from 40 to 550 nm into two biofilms, Burkholderia multivorans LMG 18825 and Pseudomonas aeruginosa LMG 27622, was evaluated using confocal microscopy. Through image analysis, the percentage of particles able to penetrate into dense biofilm clusters was calculated. The size cut off for optimal penetration into biofilm clusters was located around 100-130 nm for both biofilms. The mesh size of the biofilm matrix and the size of the channels in between the bacteria of the clusters are two factors which likely play a role in the exclusion of the larger particles. For B. multivorans, a sharp drop in the penetration into the clusters is seen for particles larger than 130 nm while for P. aeruginosa, a more gradual decrease in penetration could be observed. The overall penetration of the nanoparticles was slightly lower for P. aeruginosa than for B. multivorans.Based on these results, it could be concluded that nanocarriers of about 100 nm and smaller are good candidates to improve the treatment of chronic pulmonary biofilms in CF patients. Furthermore, the confocal microscopy method demonstrated here is a useful tool to assess the penetration of nanomedicines in biofilm clusters. Such information is important to optimize nanomedicine formulations for the treatment of biofilm infections. (C) 2014 Elsevier B.V. All rights reserved.