Flexible shape-memory scaffold for minimally invasive delivery of functional tissues
Flexible shape-memory scaffold for minimally invasive delivery of functional tissues
复制标题
DOI：
10.1038/nmat4956
复制
发表时间：
2017-10-01
期刊：
影响因子：
41.2
通讯作者：
Radisic, Milica
中科院分区：
文献类型：
--
作者：
Montgomery, Miles;Ahadian, Samad;Radisic, Milica
尽管用于器官修复（包括心脏）的功能组织工程学取得了很大进展，但它们的植入仍然需要侵入性手术方法。在这里，我们设计了一种弹性和微制造支架，使用可生物降解的聚合物（聚（辛亚甲基马来酸（酸酐）柠檬酸盐））通过注射功能组织交付。支架的形状记忆是由于微加工的晶格设计。支架和心脏补片（1 cm x 1 cm）通过小至1 mm的孔输送，在注射后恢复其初始形状，而不影响心肌细胞活力和功能。在皮下同系大鼠模型中，当比较血管形成、巨噬细胞募集和细胞存活时，注射心脏补片等同于开放手术。与未经处理的对照组相比，贴片显著改善了大鼠心肌梗死后的心脏功能。在大型动物（猪）模型中，成功地将人细胞衍生的补片微创递送至心外膜、主动脉和肝脏。
Despite great progress in engineering functional tissues for organ repair, including the heart, an invasive surgical approach is still required for their implantation. Here, we designed an elastic and microfabricated scaffold using a biodegradable polymer (poly(octamethylene maleate (anhydride) citrate)) for functional tissue delivery via injection. The scaffold's shape memory was due to the microfabricated lattice design. Scaffolds and cardiac patches (1 cm x 1 cm) were delivered through an orifice as small as 1 mm, recovering their initial shape following injection without affecting cardiomyocyte viability and function. In a subcutaneous syngeneic rat model, injection of cardiac patches was equivalent to open surgery when comparing vascularization, macrophage recruitment and cell survival. The patches significantly improved cardiac function following myocardial infarction in a rat, compared with the untreated controls. Successful minimally invasive delivery of human cell-derived patches to the epicardium, aorta and liver in a large-animal (porcine) model was achieved.