Cell transfer printing from patterned poly (ethylene glycol)-oleyl surfaces to biological hydrogels for rapid and efficient cell micropatterning
Cell transfer printing from patterned poly (ethylene glycol)-oleyl surfaces to biological hydrogels for rapid and efficient cell micropatterning
复制标题
从图案化聚乙二醇油基表面到生物水凝胶的细胞转移印刷，以实现快速高效的细胞微图案化
DOI：
10.1002/bit.23284
复制
发表时间：
2011
影响因子：
3.8
通讯作者：
et al
中科院分区：
文献类型：
--
作者：
T.Takano;et al
本文研究了从图案化的聚（乙二醇）-油基表面到生物水凝胶片上的细胞转印，作为用于细胞微阵列和组织工程的新的细胞压印方法。通过将水凝胶片覆盖在固定在聚（乙二醇）-油基表面上的细胞上并连续将其剥离，将固定的细胞转移到水凝胶片上，因为细胞与水凝胶之间的粘附相互作用比细胞与聚（乙二醇）-油基表面之间的粘附相互作用更强。四种类型的人类细胞可以有效地转移到刚性胶原蛋白片上。所有电池的转印率均在80%以上，并在90分钟内实现。利用本发明的压印方法成功地在胶原凝胶片上制备了细胞微阵列。我们还证明了内皮细胞的转移模式转化为重构基底膜基质上的图案化管状结构。最后，两种类型的内皮细胞的图案被证明很容易在基质上制备，并且自发地形成了所需的管状结构，包括两种不同细胞的有序图案。因此，本发明的聚（乙二醇）-油基包被的基底可用于在细胞外基质上制备多细胞模式中快速和有效的细胞冲压。Biotechnol. Bioeng. 2012;109：244-251。© 2011 Wiley Periodicals，Inc.
Cell transfer printing from patterned poly(ethylene glycol)‐oleyl surfaces onto biological hydrogel sheets is investigated herein, as a new cell stamping method for both cell microarray and tissue engineering. By overlaying a hydrogel sheet on the cells immobilized on the poly(ethylene glycol)‐oleyl surface and successively peeling it off, the immobilized cells were transferred onto a hydrogel sheet because the adhesive interaction between the cells and the hydrogel was stronger than that between the cells and the poly(ethylene glycol)‐oleyl surface. Four types of human cell could be efficiently transferred onto a rigid collagen sheet. The transfer printing ratios, for all cells, were above 80% and achieved within 90 min. A cell microarray was successfully prepared on a collagen gel sheet using the present stamping method. We have also demonstrated that the transferred pattern of endothelial cells is transformed to the patterned tube‐like structure on the reconstituted basement membrane matrix. Finally, the patterns of two types of endothelial cell are shown to be easily prepared on the matrix, and the desired tube‐like structures, including the orderly pattern of the two different cells, were formed spontaneously. Thus, the present poly(ethylene glycol)‐oleyl coated substrates are useful for rapid and efficient cell stamping, in the preparation of multi‐cellular pattern on extracellular matrices. Biotechnol. Bioeng. 2012;109: 244–251. © 2011 Wiley Periodicals, Inc.