The transmembrane protein FgSho1 regulates fungal development and pathogenicity via the MAPK module Ste50-Ste11-Ste7 in Fusarium graminearum
The transmembrane protein FgSho1 regulates fungal development and pathogenicity via the MAPK module Ste50-Ste11-Ste7 in Fusarium graminearum
复制标题
跨膜蛋白 FgSho1 通过 MAPK 模块 Ste50-Ste11-Ste7 调节禾谷镰刀菌中的真菌发育和致病性
DOI：
10.1111/nph.13158
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发表时间：
2015-04-01
期刊：
影响因子：
9.4
通讯作者：
Ma, Zhonghua
中科院分区：
文献类型：
--
作者：
Gu, Qin;Chen, Yun;Ma, Zhonghua
丝裂原活化蛋白激酶（MAPK）信号通路的特点是在禾谷镰刀菌。目前，这些途径的上游传感器是未知的。利用目的基因缺失策略研究了跨膜蛋白FgSho 1的生物学功能。深入分析了FgSho 1与MAPK表达盒FgSte 50-Ste 11-Ste 7之间的关系。跨膜蛋白FgSho 1是禾谷镰刀菌分生孢子形成、全毒力和脱氧雪腐镰刀菌烯醇（DON）生物合成所必需的。此外，FgSho 1和FgSln 1对禾谷镰刀菌的毒力具有加性效应。酵母双杂交，共免疫沉淀，共定位和亲和捕获质谱分析强烈表明，FgSho 1与MAPK模块FgSte 50-Ste 11-Ste 7物理相互作用。与FgSho 1突变体相似，FgSte 50、FgSte 11和FgSte 7突变体在产孢、致病性和DON生物合成方面都有缺陷。此外，FgSho 1起着次要的作用，在响应渗透胁迫，但它参与细胞壁完整性途径，这是独立的模块FgSte 50-Ste 11-Ste 7在禾谷镰刀菌。总的来说，这项研究的结果强烈表明，FgSho 1通过MAPK模块FgSte 50-Ste 11-Ste 7在禾谷镰刀菌中调节真菌发育和致病性，这与芽殖酵母酿酒酵母中已知的不同。
The mitogen-activated protein kinase (MAPK) signaling pathways have been characterized in Fusarium graminearum. Currently, the upstream sensors of these pathways are unknown. Biological functions of a transmembrane protein FgSho1 were investigated using a target gene deletion strategy. The relationship between FgSho1 and the MAPK cassette FgSte50-Ste11-Ste7 was analyzed in depth. The transmembrane protein FgSho1 is required for conidiation, full virulence, and deoxynivalenol (DON) biosynthesis in F.graminearum. Furthermore, FgSho1 and FgSln1 have an additive effect on virulence of F.graminearum. The yeast two-hybrid, coimmunoprecipitation, colocalization and affinity capture-mass spectrometry analyses strongly indicated that FgSho1 physically interacts with the MAPK module FgSte50-Ste11-Ste7. Similar to the FgSho1 mutant, the mutants of FgSte50, FgSte11, and FgSte7 were defective in conidiation, pathogenicity, and DON biosynthesis. In addition, FgSho1 plays a minor role in the response to osmotic stress but it is involved in the cell wall integrity pathway, which is independent of the module FgSte50-Ste11-Ste7 in F.graminearum. Collectively, results of this study strongly indicate that FgSho1 regulates fungal development and pathogenicity via the MAPK module FgSte50-Ste11-Ste7 in F.graminearum, which is different from what is known in the budding yeast Saccharomyces cerevisiae.