Changes of Short-Chain Fatty Acids and Their Receptors in an Obese Rat Model After Sleeve Gastrectomy
Changes of Short-Chain Fatty Acids and Their Receptors in an Obese Rat Model After Sleeve Gastrectomy
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DOI：
10.1007/s11695-022-06130-9
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发表时间：
2022-06-01
期刊：
影响因子：
2.9
通讯作者：
Inomata, Masafumi
中科院分区：
文献类型：
--
作者：
Fujinaga, Atsuro;Ohta, Masayuki;Inomata, Masafumi
背景短链脂肪酸（SCFAs）和肠道微生物群具有与健康相关的影响，并与多种疾病相关。然而，胃袖状切除术（SG）后SCFAs及其受体的变化尚不清楚。方法将30只肥胖SD大鼠随机分为假手术对照组（SO）、配对喂养对照组（PF）和SG组，每组10只。术后6周检测各组大鼠代谢指标、血和粪便中SCFA水平、回肠和附睾脂肪中SCFA受体mRNA和蛋白表达以及肠道菌群。SG组血液和粪便中的乙酸水平显著高于PF组。SG组粪便中的丁酸水平也显著高于PF组。在回肠和附睾脂肪中，SG组GPR41的mRNA和蛋白表达显著高于其他两组，SG组GPR43的mRNA和蛋白表达显著高于PF组。SG后粪便中肠球菌属、乳杆菌属、乳球菌属和梭菌属的数量增加。结论SG可能通过改变肠道菌群激活SCFA通路。
Background Short-chain fatty acids (SCFAs) and gut microbiota have health-related effects and are associated with a wide range of disorders. However, the changes of SCFAs and their receptors after sleeve gastrectomy (SG) remain unclear. This study aimed to examine changes of SCFAs and their receptors after SG in an obese rat model.Methods Thirty obese Sprague-Dawley rats eating a high-energy diet for 6 weeks were divided into three groups: sham-operated (SO) control, pair-fed (PF) control, and SG group. Six weeks after the surgery, metabolic parameters, SCFA levels in the blood and stool, mRNA and protein expression of SCFA receptors in the ileum and epididymal fat, and gut microbiota were examined.Results Metabolic parameters in the SG group were significantly improved compared with the SO group. Acetic acid levels in the blood and stool were significantly higher in the SG group than the PF group. The butyric acid level in the stool was also significantly higher in the SG group than in the PF group. In the ileum and epididymal fat, mRNA and protein expression of GPR41 was significantly higher in the SG group than in the other two groups, and mRNA and protein expression of GPR43 was significantly higher in the SG group than in the PF group. Increases in the genera Enterococcus, Lactobacillus, Lactococcus, and Clostridium were observed in the stool after SG.Conclusions SG may activate SCFA pathways through a change in gut microbiota.