A forest bathing trip increases human natural killer activity and expression of anti-cancer proteins in female subjects.
A forest bathing trip increases human natural killer activity and expression of anti-cancer proteins in female subjects.
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发表时间：
2008
影响因子：
3.2
通讯作者：
Qing Li;Kanehisa Morimoto;M. Kobayashi;H. Inagaki;M. Katsumata;Y. Hirata;K. Hirata;T. Shimizu;Y. Li;Y. Wakayama;Tomoyuki Kawada;Tatsuro Ohira;Norimasa Takayama;T. Kagawa;Y. Miyazaki
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作者：
Qing Li;Kanehisa Morimoto;M. Kobayashi;H. Inagaki;M. Katsumata;Y. Hirata;K. Hirata;T. Shimizu;Y. Li;Y. Wakayama;Tomoyuki Kawada;Tatsuro Ohira;Norimasa Takayama;T. Kagawa;Y. Miyazaki
我们之前曾报道，森林沐浴旅行增强了人的NK活性、NK细胞数量和淋巴细胞中的细胞内抗癌蛋白，并且在男性受试者中，这种增加的NK活性在旅行后持续了7天以上。在本研究中，我们调查了森林沐浴旅行对女性受试者NK活性的影响。健康护士13名，年龄25~43岁，职业生涯4~18年，知情同意。受试者经历了一次为期三天/两夜的森林之旅。第一天，受试者下午在一片林地步行两个小时；第二天，受试者在两块不同的林地上、下午各走两个小时；第三天，受试者结束旅程，在抽血并完成调查问卷后返回东京。在旅行的第二天和第三天，以及旅行后的第七天和第三十天采集血液和尿样。测定血样中NK细胞活性、NK细胞、T细胞数量、颗粒溶素、穿孔素、颗粒酶A/B表达淋巴细胞数、血清雌二醇、孕酮浓度及尿液肾上腺素、去甲肾上腺素浓度。在正常工作日的跳闸前，也进行了类似的控制测量。测定了森林中植物杀菌剂的浓度。森林浴可显著提高NK细胞活性和NK、穿孔素、颗粒溶素、颗粒酶A/B表达细胞数，显著降低T细胞百分率和尿中肾上腺素、去甲肾上腺素浓度。旅行后NK活性升高持续7天以上。在森林空气中检测到了α-品烯和β-品烯等植物杀菌剂。这些发现表明，森林沐浴之旅也增加了女性受试者的NK活性、NK细胞数量和细胞内抗癌蛋白水平，并且这种效果至少在旅行后7天内保持。树木释放的植物杀菌剂和胁迫激素水平的降低可能是导致NK活性增加的部分原因。
We previously reported that forest bathing trips enhanced human NK activity, number of NK cells, and intracellular anti-cancer proteins in lymphocytes, and that the increased NK activity lasted for more than 7 days after the trip in male subjects. In the present study, we investigated the effect of forest bathing trip on human NK activity in female subjects. Thirteen healthy nurses, age 25-43 years, professional career 4-18 years, were selected with informed consent. The subjects experienced a three-day/two-night trip to forest fields. On day 1, the subjects walked for two hours in the afternoon in a forest field; on day 2, they walked for two hours each in the morning and afternoon in two different forest fields; and on day 3, the subjects finished the trip and returned to Tokyo after drawing blood and completing a questionnaire. Blood and urine were sampled on the second and third days during the trip, and on days 7 and 30 after the trip. NK activity, numbers of NK and T cells, and granulysin, perforin, and granzymes A/B-expressing lymphocytes in the blood samples, the concentrations of estradiol and progesterone in serum, and the concentrations of adrenaline and noradrenaline in urine were measured. Similar control measurements were made before the trip on a normal working day. The concentrations of phytoncides in the forests were measured. The forest bathing trip significantly increased NK activity and the numbers of NK, perforin, granulysin, and granzymes A/B-expressing cells and significantly decreased the percentage of T cells, and the concentrations of adrenaline and noradrenaline in urine. The increased NK activity lasted for more than 7 days after the trip. Phytoncides, such as alpha-pinene and beta-pinene were detected in forest air. These findings indicate that a forest bathing trip also increased NK activity, number of NK cells, and levels of intracellular anti-cancer proteins in female subjects, and that this effect lasted at least 7 days after the trip. Phytoncides released from trees and decreased stress hormone levels may partially contribute to the increased NK activity.