Transgenic plants of turfgrass (Agrostis palustris Huds.) from microprojectile bombardment of embryogenic callus
Transgenic plants of turfgrass (Agrostis palustris Huds.) from microprojectile bombardment of embryogenic callus
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来自胚性愈伤组织的微粒轰击的草坪草（Agrostis palustris Huds.）转基因植物
DOI：
10.1007/bf00232305
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发表时间：
1993
影响因子：
6.2
通讯作者：
M. Sticklen
中科院分区：
文献类型：
--
作者：
H. Zhong;M. Bolyard;C. Srinivasan;M. Sticklen
转基因藤本草(Agrostis palustris Huds.，cv.Pencross；Poaceae)胚性愈伤组织经基因枪击和再生，获得了水稻肌动蛋白15‘调控序列控制下的β-葡萄糖醛酸苷酶(GUS)基因。经聚合酶链式反应(PCR)扩增和限制性内切酶消化后的4株转基因植株的基因组DNA Southern分析表明，GUS片段已被破译整合。Northern印迹分析证实，其中一株转基因植株表达了GUS基因。Western印迹分析显示GUS蛋白有较高水平的积累。组织化学检测表明，转基因草坪植株各部位均有β-葡萄糖醛酸酶活性。GUS在转基因植株不同组织中的表达水平大小顺序为：茎节>；第一幼叶>；根尖>；第二/第三/第四幼叶>；茎节间>；根毛区。
Transgenic creeping bentgrass (Agrostis palustris Huds., cv. Pencross; Poaceae) plants have been obtained by microprojectile bombardment of and regeneration from embryogenic calli with a vector designed to deliver the β-glucuronidase (GUS) gene under the control of rice actin 1 5' regulatory sequences. Southern analysis of polymerase chain reaction (PCR)-amplified and restriction-digested genomic DNA of four transgenic plants regenerated from these cultures showed the unscrambled integration of the gus fragment. Northern blot analysis confirmed the expression of gus mRNA in one of the transgenic plants. Western blot analysis revealed a high level of accumulation of gus protein. Histochemical assays showed enzymatic activity of β-glucuronidase in all parts of the transgenic turfgrass plant. The order of gus expression level in different tissues of the transgenic plant is as follows: stem node > first young leaf > root tip > second / third / fourth young leaf > stem internode > root hair-zone.