Culture of cells derived from the human sebaceous gland under serum-free conditions without a biological feeder layer or specific matrices
Culture of cells derived from the human sebaceous gland under serum-free conditions without a biological feeder layer or specific matrices
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DOI：
10.1007/s004030050127
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发表时间：
1996-10-01
影响因子：
3
通讯作者：
Arase, S
中科院分区：
文献类型：
--
作者：
Fujie, T;Shikiji, T;Arase, S
我们成功地在无血清条件下连续培养人皮脂腺细胞（皮脂腺细胞）。从面部皮肤标本中分离皮脂腺，在KGM中采用外植体培养和分散细胞培养两种不同的方法进行培养。两种培养方法均能使皮脂细胞在无生物饲养层和特异性基质的情况下快速增殖，可连续培养至少3代（外植体培养）和6代（分散细胞培养），并可在液氮中保存，回收率好。薄层色谱分析结果表明，细胞在体外合成了大量皮脂特异性脂质、角鲨烯和蜡酯。睾酮和sa -二氢睾酮都能显著刺激皮脂腺细胞的增殖，本文提出的方法可能允许对皮脂腺或皮脂腺细胞的功能进行大量的系列研究。
We succeeded in serially culturing cells derived from human sebaceous gland (sebocytes) under serum-free conditions. Sebaceous glands were isolated from dispase-treated facial skin specimens and cultured using two different methods, explant culture and dispersed cell culture, in KGM. In both types of culture the sebocytes proliferated rapidly without a biological feeder layer or specific matrices, It was possible to cultivate the cells serially for at least three passages (explant culture) and six passages (dispersed cell culture), and to store the cells in liquid nitrogen with good recovery, Analytical thin-layer chromatography revealed that the cells synthesized a large amount of sebum-specific lipids, squalene and wax esters in vitro. Both testosterone and Sa-dihydrotestosterone significantly stimulated the proliferation of the sebocytes, The methods presented here may permit a large series of studies on the function of sebaceous glands or cells derived from sebaceous glands.