Genetic Diversity of Porcine Epidemic Diarrhea Virus With a Naturally Occurring Truncated ORF3 Gene Found in Guangxi, China
Genetic Diversity of Porcine Epidemic Diarrhea Virus With a Naturally Occurring Truncated ORF3 Gene Found in Guangxi, China
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DOI：
10.3389/fvets.2020.00435
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发表时间：
2020-07-24
影响因子：
3.2
通讯作者：
Ouyang, Kang
中科院分区：
文献类型：
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作者：
Lu, Ying;Su, Xueli;Ouyang, Kang
猪流行性腹泻病毒（Porcine epidemic diarrhea virus，PEDV）是猪肠道的主要病原之一，引起严重的肠道疾病，造成巨大的经济损失。ORF 3基因编码一种与PEDV的感染性和毒力相关的辅助蛋白。本研究于2017 - 2019年在广西采集了33份PEDV阳性田间样品，并对ORF 3基因进行了遗传多样性分析。共获得38株ORF 3，其中5株ORF 3为广西天然截短株，全长293 bp，连续缺失172 ~ 554 bp。广西自然截短菌株编码一个89个氨基酸的截短蛋白，并聚成一个新的类群，称为类群3，这可能与毒力变异有关。对ORF 3基因的遗传和进化分析表明，广西地区同时存在G1-1亚群、G1-3亚群和G3亚群3种基因型。本研究中的序列信息将有助于建立一种能够区分PEDV野毒株的诊断方法。持续监测将有助于监测PEDV传播。对PEDV野毒株ORF 3基因的差异分析，有助于进一步筛选PEDV疫苗候选株，更有效地预防PED的暴发流行。
Porcine epidemic diarrhea virus (PEDV) is one of the major enteric pathogens, causing severe enteric disease, resulting in enormous economic losses. The ORF3 gene encodes an accessory protein which is related to the infectivity and virulence of PEDV. In this study, 33 PEDV positive field samples were collected from Guangxi, from 2017 to 2019, and the genetic diversity of ORF3 was investigated. Thirty-eight strains of ORF3 were obtained, and these were composed of five strains of ORF3 named Guangxi naturally truncated strains that were 293 bp in length, with continuous deletions from 172 to 554 bp. The Guangxi naturally truncated strains encoded a truncated protein of 89 amino acids, which had clustered into a new group referred to as Group 3, and these might be involved in the variations of virulence. Three genotypes (G1-1 subgroup, G1-3 subgroup, and Group 3) existed simultaneously in Guangxi based on the genetic and evolutionary analysis of the ORF3 gene. The sequence information in the current study will hopefully facilitate the establishment of a diagnostic method that can differentiate the PEDV field stains. Continued surveillance will be useful for monitoring PEDV transmission. Differentiation of the ORF3 genes in PEDV field strains can help us to choose an appropriate PEDV vaccine candidate in the future and prevent outbreaks of PED more effectively.