Fibrin is a regulator of Schwann cell migration after sciatic nerve injury in mice
Fibrin is a regulator of Schwann cell migration after sciatic nerve injury in mice
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DOI：
10.1016/s0304-3940(02)01387-3
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发表时间：
2003-03-06
影响因子：
2.5
通讯作者：
Strickland, S
中科院分区：
文献类型：
--
作者：
Akassoglou, K;Akpinar, P;Strickland, S
纤维蛋白原来源于血液中的纤维蛋白原，在损伤后沉积在坐骨神经中并阻碍功能再生。由于雪旺细胞迁移是至关重要的损伤神经的髓鞘再生，我们研究了纤维蛋白在这个过程中的作用。在体实验表明，纤维蛋白共定位与纤维连接蛋白沉积在损伤的坐骨神经。体外迁移试验表明，单独的纤维蛋白不是许旺细胞迁移的允许底物。此外，雪旺细胞在纤维蛋白/纤连蛋白混合基质上的迁移试验表明，纤维蛋白对雪旺细胞在纤连蛋白上的迁移具有剂量依赖性抑制作用。我们的研究结果表明，纤维蛋白，损伤后沉积在坐骨神经，改变细胞外基质的组成，并抑制雪旺细胞迁移。在基于纤维蛋白基质的生物材料的治疗应用中，应考虑纤维蛋白的这种负面影响。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Fibrin, derived from the blood protein fibrinogen, is deposited in the sciatic nerve after injury and retards functional regeneration. Since Schwann cell migration is critical for remyelination of injured nerves, we investigated the effects of fibrin in this process. In vivo experiments showed that fibrin co-localizes with fibronectin deposition in the injured sciatic nerve. In vitro migration assays demonstrated that fibrin alone is not a permissive substrate for Schwann cell migration. Furthermore, migration assays of Schwann cells on mixed fibrin/fibronectin substrates showed that fibrin has a dose dependent inhibitory effect on Schwann cell migration on fibronectin. Our results show that fibrin, deposited in the sciatic nerve after injury, changes the composition of the extracellular matrix and inhibits Schwann cell migration. This negative effect of fibrin should be considered in the therapeutic application of biomaterials based on fibrin matrices. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.