Functional expression of sodium iodide symporter (NIS) in human breast cancer tissue
Functional expression of sodium iodide symporter (NIS) in human breast cancer tissue
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DOI：
10.1023/a:1021321409159
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发表时间：
2003-01-01
影响因子：
3.8
通讯作者：
Godbole, MM
中科院分区：
文献类型：
--
作者：
Upadhyay, G;Singh, R;Godbole, MM
碘化钠同向转运体（NIS）是一种参与甲状腺中碘主动积累以促进甲状腺激素生物合成的分子。它的表达也在甲状腺外组织中得到证实，包括哺乳小鼠乳腺和人类乳腺癌。甲状腺细胞内碘化物通过NIS转运是利用放射性碘诊断和治疗分化型甲状腺癌的基础。如果恶性肿瘤中碘的摄取、保留和 NIS 表达能够明确显示，那么类似的方法可能对乳腺癌的诊断和治疗有益。本研究的目的是研究人类乳腺肿瘤中 NIS 的表达、体内碘转运能力和碘的命运。本研究的对象是经 FNAC 和随后的组织病理学证实患有浸润性导管癌的女性（年龄 33-58 岁）。在手术切除的乳腺肿瘤组织中，分别通过 RNAase 保护测定、蛋白质印迹和免疫组织化学证实了 NIS RNA 和蛋白质的表达。在体内和体外评估了碘转运能力及其性质。我们报告了 NIS 在转录和翻译水平上的高表达及其在人类乳腺肿瘤中转运碘的能力。通过闪烁扫描证实了体内碘转运能力。与甲状腺不同，高氯酸盐和硫氰酸盐不会抑制乳腺肿瘤中的碘转运。碘化蛋白质的存在表明保留时间较长。 NIS表达、其功能和组织对碘的保留的明确证明进一步为使用放射性碘作为人类乳腺癌的额外治疗方式提供了支持性证据。
Sodium iodide symporter (NIS) is a molecule involved in active accumulation of iodine in thyroid gland for the biosynthesis of thyroid hormone. Its expression has also been demonstrated in extra-thyroidal tissues including lactating mice mammary gland and also in human breast cancers. Iodide transport in thyroid cells through NIS is the basis for using radioiodine for diagnosis and treatment of differentiated thyroid carcinoma. The similar approach may prove beneficial for the diagnosis and treatment of breast cancer if iodine uptake, its retention and NIS expression can be shown unequivocally in malignant tumors. The aim of the present study was to investigate NIS expression, in vivo iodine transport ability and fate of iodine in human breast tumors. Women (age 33-58 years) with infiltrating duct carcinoma confirmed by FNAC and subsequent histopathology were the subject of this study. Expression of NIS RNA and protein was confirmed by RNAase protection assay, western blot and immunohistochemistry respectively in surgically excised breast tumor tissue. Iodine transport ability and its nature was assessed both in vivo and in vitro. We report high NIS expression at both transcriptional and translational level and its ability to transport iodine in human breast tumors. The in vivo iodine transport ability was confirmed by scintigraphy. Unlike thyroid, perchlorate and thiocyanate do not inhibit iodine transport in breast tumors. The presence of iodinated proteins suggests the longer retention time. The unequivocal demonstration of NIS expression, its functionality and retention of iodine by organification further provides supportive evidence for use of radioiodine as an additional treatment modality of human breast carcinoma.