Abnormalities in rod photoreceptors, amacrine cells, and horizontal cells in human retinas with retinitis pigmentosa
Abnormalities in rod photoreceptors, amacrine cells, and horizontal cells in human retinas with retinitis pigmentosa
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DOI：
10.1016/s0002-9394(99)00401-8
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发表时间：
2000-02-01
影响因子：
4.2
通讯作者：
Milam, AH
中科院分区：
文献类型：
--
作者：
Fariss, RN;Li, ZY;Milam, AH
目的：为了评估色素性视网膜炎患者视网膜中杆状细胞、无突细胞和水平细胞的变化，方法：对来自色素性视网膜炎患者供体的7个视网膜和14个年龄和死后匹配的正常人视网膜进行免疫细胞化学和共聚焦显微镜处理。使用以下细胞特异性抗体：抗视紫红质（棒状细胞）、抗-氨基丁酸（无痘细胞）、抗白蛋白（锥体细胞和水平细胞）、抗胶质纤维酸性蛋白（星形胶质细胞和反应性Muller细胞）、抗突触素和抗sv2（突触囊泡）。结果：在光感受器明显丧失的视网膜区域，杆状细胞、γ -氨基丁酸阳性的无突细胞和卡联蛋白阳性的水平细胞出现了神经突的萌发，杆状细胞、无突细胞和水平细胞的神经突与胶质原纤维酸性蛋白免疫反应性Muller细胞的表面相关。大多数投射到视网膜内的杆状神经突与γ -氨基丁酸阳性无突细胞的体细胞接触。结论：视网膜色素性视网膜炎患者视网膜中杆状细胞、无突细胞和水平细胞发生神经突萌发。视网膜神经元的这些改变可能导致视网膜电图异常和进行性视力下降，这些改变也可能使视网膜色素变性的治疗策略复杂化（美国眼科杂志2000；124:215-223,(C) 2000 by Elsevier Science Inc.）。版权所有。)
PURPOSE: To evaluate changes in the rods and amacrine cells and horizontal cells in human retinas with retinitis pigmentosa,METHODS: Seven retinas from patient donors with retinitis pigmentosa and 14 age- and postmortem-matched normal human retinas were processed for immunocytochemistry and confocal microscopy. The following cell-specific antibodies were used: anti-rhodopsin (rods), anti-gamma-aminobutyric acid (amacrine cells), anticalbindin (cones and horizontal cells), anti-glial fibrillary acidic protein (astrocytes and reactive Muller cells), and anti-synaptophysin and anti-SV2 (synaptic vesicles).RESULTS: In retinal regions with significant photoreceptor loss, the rods, gamma-aminobutyric acid-positive amacrine cells, and calbindin-positive horizontal cells had undergone neurite sprouting, The rod, amacrine and horizontal cell neurites were associated with the surfaces of glial fibrillary acidic protein-immunoreactive Muller cells. Most rod neurites that projected into the inner retina contacted the somata of gamma-aminobutyric acid-positive amacrine cells.CONCLUSIONS: Rods, amacrine and horizontal cells undergo neurite sprouting in human retinas with retinitis pigmentosa. These changes in the retinal neurons may contribute to the electroretinographic abnormalities and progressive decline in vision noted by patients with retinitis pigmentosa, These alterations may also complicate strategies for treatment of retinitis pigmentosa, (Am J Ophthalmol 2000;129:215-223, (C) 2000 by Elsevier Science Inc. All rights reserved.)