As yet uncultured bacteria phylogenetically classified as Prevotella, Lachnospiraceae incertae sedis and unclassified Bacteroidales, Clostridiales and Ruminococcaceae may play a predominant role in ruminal biohydrogenation
As yet uncultured bacteria phylogenetically classified as Prevotella, Lachnospiraceae incertae sedis and unclassified Bacteroidales, Clostridiales and Ruminococcaceae may play a predominant role in ruminal biohydrogenation
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DOI：
10.1111/j.1462-2920.2011.02452.x
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发表时间：
2011-06-01
影响因子：
5.1
通讯作者：
Scollan, Nigel D.
中科院分区：
文献类型：
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作者：
Huws, Sharon A.;Kim, Eun J.;Scollan, Nigel D.
瘤胃中多不饱和脂肪酸（PUFA）被微生物生物加氢转化为饱和脂肪酸（SFA），导致反刍动物产品（如肉和奶）中SFA/PUFA比例较高。在体外，与proteoclasticus相关的Butyrivibrio细菌广泛地将PUFA生物氢化为SFA，但它们在瘤胃中的作用尚未得到证实。本研究的目的是评价变形破丁酸弧菌群细菌在瘤胃生物氢化中的作用，并评估其他细菌可能的作用。以干物质采食量为0%、1.5%和3%的鱼油饲喂8头荷斯坦×弗里西亚阉牛，观察其PUFA生物氢化程度的变化。测定瘤胃食糜中脂肪酸和破壁芽孢杆菌组16S rRNA浓度。食糜18:0浓度与B. proteoclasticus组16S rRNA浓度相关性较低。末端限制片段长度多态性和变性梯度凝胶电泳（DGGE）结合多元统计结果显示，许多末端限制片段（t - rf）和DGGE条带与顺式-9、反式-11共轭亚油酸（CLA）、18:1反式-11和18:0瘤胃浓度相关。MiCA T-RF预测鉴定软件显示，这些相关的T-RF可能来自尚未培养的细菌，分类为Prevotella， Lachnospiraceae incertae sedis，以及未分类的Bacteroidales， Clostridiales和Ruminococcaceae。DGGE序列还显示，尚未培养的普雷沃菌属、厌氧菌属（毛螺杆菌科）、未分类的梭菌属和瘤胃球菌科可能在生物加氢过程中发挥作用。
Microbial biohydrogenation of dietary polyunsaturated fatty acids (PUFA) to saturated fatty acids (SFA) in the rumen results in the high ratio of SFA/PUFA in ruminant products, such as meat and milk. In vitro, Butyrivibrio proteoclasticus-related bacteria extensively biohydrogenate PUFA to SFA, yet their contribution in the rumen has not been confirmed. The aim of this study was to evaluate the role of Butyrivibrio proteoclasticus group bacteria in ruminal biohydrogenation and to assess the possible role of other bacteria. Fish oil at 0%, 1.5% and 3% dry matter intake was fed to eight Holstein x Friesian steers, in order to elicit changes in the extent of PUFA biohydrogenation. Fatty acid and B. proteoclasticus group 16S rRNA concentrations in rumen digesta were determined. Correlation between digesta 18: 0 concentration and B. proteoclasticus group 16S rRNA concentration was low. Terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis (DGGE) coupled with multivariate statistics revealed that many terminal restriction fragments (T-RFs) and DGGE bands were linked to cis-9, trans-11 conjugated linoleic acid (CLA), 18: 1 trans-11 and 18: 0 ruminal concentrations. MiCA T-RF predictive identification software showed that these linked T-RFs were likely to originate from as yet uncultured bacteria classified as Prevotella, Lachnospiraceae incertae sedis, and unclassified Bacteroidales, Clostridiales and Ruminococcaceae. Sequencing of linked DGGE bands also revealed that as yet uncultured bacteria classified as Prevotella, Anaerovoax (member of the Lachnospiraceae incertae sedis family), and unclassified Clostridiales and Ruminococcaceae may play a role in biohydrogenation.