Retinal capillary basement membrane thickening in a porcine model of diabetes mellitus.
Retinal capillary basement membrane thickening in a porcine model of diabetes mellitus.
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发表时间：
2002-12
影响因子：
0.8
通讯作者：
D. Hainsworth;M. Katz;Deja A Sanders;D. N. Sanders;Ethan J Wright;M. Sturek
中科院分区：
文献类型：
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作者：
D. Hainsworth;M. Katz;Deja A Sanders;D. N. Sanders;Ethan J Wright;M. Sturek
目的目前包括糖尿病视网膜病变(DR)在内的糖尿病微血管病变的动物模型有很大的局限性。为了确定糖尿病猪是否会提供一种改进的大型糖尿病动物模型，对20周的糖尿病猪模型进行了评估，该模型表现出明显的大血管病变，并与人类糖尿病(以下简称糖尿病)相关的视网膜微血管改变的发生进行了评估。方法观察糖尿病单独或合并高脂饮食对视网膜毛细血管形态的影响。用弹性酶消化法分离视网膜血管床，测定视网膜毛细血管内细胞密度，并用电子显微镜分析测定视网膜毛细血管基底膜厚度。结果饲喂正常饲料的糖尿病猪视网膜毛细血管基底膜明显厚于非糖尿病猪(P<；0.001)。高脂肪摄入量不会导致基底膜增厚。没有观察到糖尿病或饮食对视网膜毛细血管细胞密度的显著影响，也没有观察到糖尿病导致晚期糖尿病视网膜病变典型的微动脉瘤的形成。结论糖尿病猪视网膜毛细血管基底膜增厚的快速发展为糖尿病相关微血管改变的早期干预和机制研究提供了良好的动物模型。
PURPOSE Current animal models of diabetic microangiopathy, including diabetic retinopathy (DR), have substantial limitations. To determine whether the diabetic pig would provide an improved large animal model of DR, a 20-week porcine model of diabetic dyslipidemia that manifests appreciable macrovasculopathy was evaluated for development of retinal microvascular changes associated with diabetes mellitus (hereafter referred to as diabetes) in humans. METHODS The effect of diabetes alone or in combination with high dietary fat intake on retinal capillary morphology was assessed. Cell density in the retinal capillaries was evaluated by use of elastase digestion isolation of the retinal vascular bed, and retinal capillary basement membrane thickness was determined by use of electron microscopic analyses. RESULTS Diabetic pigs fed a normal diet had significantly (P < 0.001) thicker retinal capillary basement membranes than did nondiabetic animals. High fat intake did not contribute to basement membrane thickening. Significant effects of diabetes or diet on retinal capillary cell densities were not observed, nor did diabetes induce formation of microaneurysms typical of advanced diabetic retinopathy. CONCLUSIONS The rapid development of retinal capillary basement membrane thickening in diabetic pigs makes this animal model useful for early intervention and mechanistic studies for this diabetes-associated microvascular change.