INTERMEDIATE FILAMENT PROTEIN AS A MARKER OF UTERINE STROMAL CELL DECIDUALIZATION
INTERMEDIATE FILAMENT PROTEIN AS A MARKER OF UTERINE STROMAL CELL DECIDUALIZATION
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DOI：
10.1095/biolreprod35.2.463
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发表时间：
1986-09-01
影响因子：
3.6
通讯作者：
JULIAN, J
中科院分区：
文献类型：
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作者：
GLASSER, SR;JULIAN, J
在体内和体外的大鼠子宫间质细胞经历蜕膜化的中间丝的增加已被报道。为了确定这种形态学变化的生化相关性，我们已经确定（二维凝胶电泳，蛋白质印迹，间接免疫荧光染色）的组成性中间丝蛋白质的基质细胞在体内和分离的基质蜕膜在体外的波形蛋白和结蛋白。波形蛋白是所有子宫间质细胞所共有的，但在蜕膜化间质中与总细胞蛋白成比例地增加。在非蜕膜化间质中几乎检测不到结蛋白，与波形蛋白不同，结蛋白在蜕膜化过程中以大于总细胞蛋白增加的速率增加。波形蛋白或结蛋白的增加在子宫致敏的非蜕膜基质细胞中均未观察到。结蛋白，因为它是选择性地表达在蜕膜基质，可以被认为是独特的足以作为一个标志物的蜕膜细胞分化。
An increase in intermediate filaments has been reported in rat uterine stromal cells undergoing decidualization in vivo and in vitro. In order to identify biochemical correlates of this morphological change, we have identified (two dimensional gel electrophoresis, Western blots, indirect immunofluorescent staining) the constitutive intermediate filament proteins of stromal cells decidualizing in vivo and isolated stroma decidualizing in vitro as vimentin and desmin. Vimentin is common to all uterine stromal cells but increases, proportional to total cell protein, in decidualized stroma. Barely detectable in nondecidualized stroma, desmin, unlike vimentin, increases during decidualization at a rate greater than the increase in total cell protein. Neither the increase in vimentin or desmin is observed in hormonally sensitized, nondecidual stromal cells. Desmin, because it is selectively expressed in decidualizing stroma, could be considered unique enough to serve as a marker of decidual cell differentiation.