Biased expression of T cell receptor Vβ genes in periodontitis patients
Biased expression of T cell receptor Vβ genes in periodontitis patients
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牙周炎患者T细胞受体Vβ基因的偏向表达
DOI：
10.1046/j.1365-2249.1996.d01-834.x
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发表时间：
1996
影响因子：
4.6
通讯作者：
K. Hara
中科院分区：
文献类型：
--
作者：
K. Yamazaki;T. Nakajima;E. Gemmell;M. Kjeldsen;G. Seymour;K. Hara
本研究的目的是研究牙周炎组织中携带T细胞受体（TCR）的有限T细胞库的选择性激活和扩增的假设。我们研究了TCR Vβ基因在牙周炎病变牙龈组织中的表达，并将其与同一患者外周血单核细胞（PBMC）的表达进行了比较，采用聚合酶链反应（PCR）扩增方法，用22个Vβ特异性引物与一个常见的反义Cβ特异性引物联合扩增。初步治疗后，收集14例牙周炎患者牙周手术时的牙龈组织标本，并采用密度梯度离心法提取PBMC。提取总RNA，用反转录PCR分析。PCR产物在2%琼脂糖凝胶上电泳，溴化乙啶染色。对于每种产品，比较牙龈组织和各自的外周血。在PBMC中发现的TCR曲目通常与牙龈组织的TCR曲目重叠。然而，与PBMC相比，Vβ6在牙龈组织中表现为过表达，而Vβ16在牙龈组织中表现为低表达。虽然尚不清楚这是由于抗原特异性激活还是超抗原激活，但数据表明牙周病组织中可能存在尚未表征的T细胞亚群。
The aim of the present study was to investigate the hypothesis that there is selective activation and expansion of a limited repertoire of T cell receptor (TCR)‐bearing T cells in periodontitis tissue. We studied TCR Vβ gene expression in gingival tissues of periodontitis lesions and compared these with peripheral blood mononuclear cells (PBMC) from the same patients using polymerase chain reaction (PCR) amplification with 22 Vβ‐specific sense primers in combination with a common antisense Cβ‐specific primer. After initial therapy, gingival tissue specimens were obtained from 14 periodontitis patients at the time of periodontal surgery, and PBMC were also obtained from the same patients by density gradient centrifugation. Total RNA was extracted and analysed by reverse transcription PCR. The PCR products were electrophoresed on a 2% agarose gel and stained with ethidium bromide. For each product, gingival tissue and the respective peripheral blood were compared. The TCR repertoire identified in the PBMC in general overlapped with that of the gingival tissue. However, Vβ6 appeared to be over‐expressed in the gingival tissues compared with the PBMC, but Vβ16 appeared to be under‐expressed in the gingival tissues. Although it is not known whether this is due to antigen‐specific activation or superantigen activation, the data suggest that there may be as yet uncharacterized T cell subsets in periodontal disease tissue.
人类利什曼病中的 CD4 1 型和 CD8 2 型 T 细胞亚群具有不同的 T 细胞受体库。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Uyemura,K;Pirmez,C;Sieling,PA;Kiene,K;Paes-Oliveira,M;Modlin,RL
通讯作者：
Modlin,RL