Bifidobacterium strains suppress in vitro the pro-inflammatory milieu triggered by the large intestinal microbiota of coeliac patients
Bifidobacterium strains suppress in vitro the pro-inflammatory milieu triggered by the large intestinal microbiota of coeliac patients
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DOI：
10.1186/1476-9255-5-19
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发表时间：
2008-11-03
影响因子：
5.1
通讯作者：
Sanz, Yolanda
中科院分区：
文献类型：
--
作者：
Medina, Marcela;De Palma, Giada;Sanz, Yolanda
背景：乳糜泻（CD）是一种肠道疾病，其特征是对谷蛋白的异常免疫反应。虽然谷蛋白肽和微生物激活类似的促炎途径，但肠道微生物群在这种疾病中可能发挥的作用尚不清楚。本研究的目的是评估乳糜泻患者的粪便微生物群是否有助于CD的促炎环境特征以及生物多样性细菌的可能益处。26例活动期CD患者粪便的影响（平均年龄5.5岁，范围2.1-12.0岁），18例无乳糜泻（SFCD）患者（平均年龄5.5岁，范围1.0-12.3岁），无麸质饮食1-2年; 20个健康的孩子（平均年龄5.3岁，范围1.8-10.8岁）对外周血单核细胞（PBMC）中细胞因子产生和表面抗原表达的诱导作用。长双歧杆菌ES 1和B.结果：活动性CD和SFCD患者的粪便代表了不平衡的微生物群，与对照样品相比，活动性CD和SFCD患者的粪便显著增加了PBMC中TNF-α的产生和CD 86的表达，而降低了IL-10细胞因子的产生和CD 4的表达。与对照组相比，活动性CD患者样品也诱导显著更高的IFN-γ产生。然而，双歧杆菌菌株抑制CD患者大肠内容物诱导的促炎细胞因子模式，并增加IL-10的产生。结论：CD患者肠道菌群可能参与了疾病的Th 1促炎环境，而B. longum ES 1和B.双歧杆菌ES 2可以逆转这些有害作用。这些发现为CD治疗提供了未来的前景。
Background: Coeliac disease ( CD) is an enteropathy characterized by an aberrant immune response to cereal-gluten proteins. Although gluten peptides and microorganisms activate similar pro-inflammatory pathways, the role the intestinal microbiota may play in this disorder is unknown. The purpose of this study was to assess whether the faecal microbiota of coeliac patients could contribute to the pro-inflammatory milieu characteristic of CD and the possible benefits of bifidobacteria.Methods: The effect of faeces of 26 CD patients with active disease ( mean age 5.5 years, range 2.1-12.0 years), 18 symptom-free coeliac disease (SFCD) patients ( mean age 5.5 years, range 1.0-12.3 years) on a gluten-free diet for 1-2 years; and 20 healthy children ( mean age 5.3 years, range 1.8-10.8 years) on induction of cytokine production and surface antigen expression in peripheral blood mononuclear cells (PBMCs) were determined. The possible regulatory roles of Bifidobacterium longum ES1 and B. bifidum ES2 co-incubated with faecal samples were also assessed in vitro.Results: Faeces of both active CD and SFCD patients, representing an imbalanced microbiota, significantly increased TNF-alpha production and CD86 expression in PBMCs, while decreased IL-10 cytokine production and CD4 expression compared with control samples. Active CD-patient samples also induced significantly higher IFN-gamma production compared with controls. However, Bifidobacterium strains suppressed the pro-inflammatory cytokine pattern induced by the large intestinal content of CD patients and increased IL-10 production. Cytokine effects induced by faecal microbiota seemed to be mediated by the NF kappa B pathway.Conclusion: The intestinal microbiota of CD patients could contribute to the Th1 pro-inflammatory milieu characteristic of the disease, while B. longum ES1 and B. bifidum ES2 could reverse these deleterious effects. These findings hold future perspectives of interest in CD therapy.