A novel model-gel-tissue assay analysis for comparing tumor elastic properties to collagen content.
A novel model-gel-tissue assay analysis for comparing tumor elastic properties to collagen content.
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一种新颖的模型凝胶组织测定分析，用于比较肿瘤弹性特性与胶原蛋白含量。
DOI：
10.1007/s10237-009-0150-9
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发表时间：
2009
影响因子：
3.5
通讯作者：
Miga,MichaelI
中科院分区：
文献类型：
--
作者：
Barnes,StephanieL;Young,PampeeP;Miga,MichaelI
在之前的工作中，实现了一种新的测定方法来确定软组织机械性能。该方法被称为模型-凝胶-组织 (MGT) 测定，将材料测试与通过凝胶嵌入组织样本的显微 CT 图像采集构建的有限元模型相结合，以确定其机械性能。鉴于最近的报告表明基质胶原蛋白的增加促进乳腺肿瘤的发生和增殖，在本文中，MGT 测定用于评估小鼠乳腺肿瘤的模量，并随后与 I 型胶原蛋白含量定量相关。此外，本文还报告了关于凝胶体积与组织质量比的测定灵敏度的初步测试。结果表明肿瘤机械特性和胶原蛋白含量之间存在很强的线性相关性 (R2= 0.9462)。这一结果很重要，因为 MGT 测定提供的机械刚度与使用弹性成像技术进行临床研究的参数非常相似。灵敏度测试表明，成功分析需要大约 16.5 ml g−1 的凝胶体积与组织质量比阈值。这是一个重要的结果，因为它提出了进行该分析的指导约束。
In previous work, a new assay was realized for determining soft-tissue mechanical properties. The method, named the model-gel-tissue (MGT) assay, couples material testing with a finite element model built from a micro-CT image acquisition of a gel-embedded tissue specimen to determine its mechanical properties. Given recent reports demonstrating that increased stromal collagen promotes mammary tumor initiation and proliferation, in this paper, the MGT assay is used to evaluate the modulus of murine mammary tumors and is subsequently correlated quantitatively to type I collagen content. In addition, preliminary testing of the assay sensitivity with respect to gel-volume to tissue-mass ratio is reported here. The results demonstrate a strong linear correlation between tumor mechanical properties and collagen content (R2= 0.9462). This result is important because mechanical stiffness as provided by the MGT assay is very similar to parameters under clinical investigation using elastographic imaging techniques. The sensitivity tests indicated that an approximate gel-volume to tissue-mass ratio threshold of 16.5 ml g−1is needed for successful analysis. This is an important result in that it presents guideline constraints for conducting this analysis.