DEMONSTRATION OF AXONAL PROJECTIONS OF NEURONS IN THE RAT HIPPOCAMPUS AND SUBICULUM BY INTRACELLULAR INJECTION OF HRP
DEMONSTRATION OF AXONAL PROJECTIONS OF NEURONS IN THE RAT HIPPOCAMPUS AND SUBICULUM BY INTRACELLULAR INJECTION OF HRP
复制标题
DOI：
10.1016/0006-8993(83)90283-4
复制
发表时间：
1983-01-01
期刊：
影响因子：
2.9
通讯作者：
BABB, TL
中科院分区：
文献类型：
--
作者：
FINCH, DM;NOWLIN, NL;BABB, TL
向大鼠的海马形成神经元细胞内注射辣根过氧化物酶，以追踪内在和外在的轴突投射。 CA3 金字塔 (n = 9) 将轴突向喙侧投射向伞毛，一根或多根 Schaffer 侧枝向 CA1 投射，在两种情况下，纤维穿过海马连合。 CA1 的金字塔（n = 5）将轴突投射到牙槽，在那里它们尾部向下托前进。一个子集（n = 3）也将轴突分支向喙部投射到菌毛。这些发现不仅证实了阿蒙角金字塔的主要目标区域，而且还强调了它们不同的轴突投影，这些投影在组织上不一定是层状的。下锥体 (n = 12) 的轴突向喙部、尾部或两个方向投射。它们可以追溯到其他几个皮质区域，特别是阿蒙角、内嗅皮质和扣带皮层。皮下神经元是海马体本身输入的接受者，也是海马结构传出信号的主要来源。 CA1 中的多过程神经元具有与抑制性中间神经元相关的生理特性，并被详细填充和追踪。它最类似于洛伦特·德·诺（Lorente de No）所描述的多角篮状细胞，具有长的径向定向树突，一直延伸到空隙分子层。空洞分子层中假定的抑制性中间神经元树突的存在表明这些树突片段除了传统的反复抑制之外还有一些作用，并且描述了两种可能的电路。
Hippocampal formation neurons of rat were injected intracellularly with horseradish peroxidase in order to trace intrinsic and extrinsic axonal projections. CA3 pyramids (n = 9) projected axons rostrally toward the fimbria, one or more Schaffer collaterals toward CA1, and in two cases fibers that crossed the hippocampal commissure. Pyramids of CA1 (n = 5) projected axons to the alveus where they proceeded caudally toward the subiculum. A subset (n = 3) also projected an axonal branch rostrally toward the fimbria. These findings confirm not only major target regions of Ammon''s horn pyramids, but also emphasize their divergent axonal projections that are not necessarily lamellar in organization. Axons from subicular pyramids (n = 12) projected rostrally, caudally, or in both directions. They could be traced to several other cortical regions, specifically Ammon''s horn, entorhinal cortex and cingulate cortex. Subicular neurons are the recipient of input from the hippocampus proper and are a principal source of efferents from the hippocampal formation. A multi-process neuron in CA1 with physiologic properties associated with inhibitory interneurons was filled and traced in detail. It most resembled the poligonal basket cells that Lorente de No described, having long radially oriented dendrites extending as far as stratum lacunosum-moleculare. The presence of putative inhibitory interneuron dendrites in stratum lacunosum-molecular suggests some role other than traditional recurrent inhibition for these dendritic segments, and 2 possible circuits are described.