The Solanum americanum pangenome and effectoromics reveal new resistance genes against potato late blight
The Solanum americanum pangenome and effectoromics reveal new resistance genes against potato late blight
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DOI：
10.1101/2022.08.11.503608
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发表时间：
2022-08
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通讯作者：
Xiao Lin;Yuxin Jia;R. Heal;Maxim Prokchorchik;M. Sindalovskaya;A. Olave-Achury;Moffat Makechemu;Sebastian Fairhead;Azka Noureen;Jung-Soo Heo;Kamil Witek;M. Smoker;Jodie Taylor;R. Shrestha;Yoonyoung Lee;Chunzhi Zhang;S. Park;K. Sohn;Sanwen Huang;Jonathan D. G. Jones
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作者：
Xiao Lin;Yuxin Jia;R. Heal;Maxim Prokchorchik;M. Sindalovskaya;A. Olave-Achury;Moffat Makechemu;Sebastian Fairhead;Azka Noureen;Jung-Soo Heo;Kamil Witek;M. Smoker;Jodie Taylor;R. Shrestha;Yoonyoung Lee;Chunzhi Zhang;S. Park;K. Sohn;Sanwen Huang;Jonathan D. G. Jones
由卵菌病原体致病疫霉（Phytophthora infestans）引起的晚疫病继续在马铃薯和番茄中造成重大的全球性损失。美洲茄（Solanumamericanum）是茄科野生植物，在世界范围内广泛分布，大多数品种对晚疫病具有高度抗性。我们生成了四个S的高质量参考基因组。americanum种质，重新测序了52份种质，我们定义了NLR免疫受体基因（S。americanum NLRome）。我们进一步筛选了52个S.美洲的加入。利用这些基因型和表型数据，我们从致病疫霉中克隆了三个新的NLR编码基因Rpi-amr 4、Rpi-amr 16和Rpi-amr 17，并确定了它们相应的RXLR效应基因Avramr 4（PITG_22825）、Avramr 16（PITG_02860）和Avramr 17（PITG_04373）。这些基因组资源和方法将支持将马铃薯转化为晚疫病的“非宿主”的努力，并可应用于其他作物的疾病。
Late blight caused by the oomycete pathogen Phytophthora infestans continues to cause major worldwide losses in potato and tomato. Most accessions of Solanum americanum, a globally distributed, wild Solanaceae plant, are highly resistant to late blight. We generated high-quality reference genomes of four S. americanum accessions, re-sequenced 52 accessions, and we defined variation in the NLR immune receptor genes (the S. americanum NLRome). We further screened for variation in recognition of ∼315 P. infestans RXLR effectors in 52 S. americanum accessions. Using these genotypic and phenotypic data, we cloned three novel NLR-encoding genes Rpi-amr4, Rpi-amr16 and Rpi-amr17, and determined their corresponding RXLR effector genes Avramr4 (PITG_22825), Avramr16 (PITG_02860) and Avramr17 (PITG_04373) from P. infestans. These genomic resources and methodology will support efforts to convert potato into a “nonhost” of late blight and can be applied to diseases of other crops.