Scanning Electrochemical Microscopy in Neuroscience
Scanning Electrochemical Microscopy in Neuroscience
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DOI：
10.1146/annurev.anchem.111808.073651
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Schuhmann, Wolfgang
中科院分区：
文献类型：
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作者：
Schulte, Albert;Nebel, Michaela;Schuhmann, Wolfgang
本文综述了扫描电化学显微镜（SECM）在单个培养活细胞研究中的应用，重点介绍了神经和内分泌系统的神经元和分泌细胞的地形和功能成像。讨论了生物SECM及其相关的负电流反馈和发生器/收集器模式SECM成像的基本原理，并概述了该方法在筛选柔软和脆弱膜性物体方面的成功应用。介绍了恒高探头运动模式的缺点和恒远探头运动模式的优点。最后，强调了各种活细胞上的恒定高度和恒定距离模式SECM的代表性例子，以展示单细胞SECM的总体现状，特别是神经科学中的SECM。
This article reviews recent work involving the application of scanning electrochemical microscopy (SECM) to the study of individual cultured living cells, with an emphasis on topographical and functional imaging of neuronal and secretory cells of the nervous and endocrine system. The basic principles of biological SECM and associated negative amperometric-feedback and generator/collector-mode SECM imaging are discussed, and successful use of the methodology for screening soft and fragile membranous objects is outlined. The drawbacks of the constant-height mode of probe movement and the benefits of the constant-distance mode of SECM operation are described. Finally, representative examples of constant-height and constant-distance mode SECM on a variety of live cells are highlighted to demonstrate the current status of single-cell SECM in general and of SECM in neuroscience in particular.