Acceleration of Skin Regeneration From Cultured Epithelial Autografts by Transplantation to Homograft Dermis
Acceleration of Skin Regeneration From Cultured Epithelial Autografts by Transplantation to Homograft Dermis
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DOI：
10.1097/00004630-199311000-00010
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
Press, William
中科院分区：
文献类型：
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作者：
Compton, Carolyn C.;Hickerson, William;Press, William
对移植到同种移植真皮的培养的自体上皮移植物在移植后9天至2.25年进行组织病理学分析，并与同一患者移植到没有同种移植真皮的创面床的培养的自体上皮移植物进行比较。结果表明，移植到真皮基质可加速培养的上皮自体移植物皮肤再生的生物学事件。在放置在真皮基质上的培养的自体上皮移植物中，网状脊的发育和表皮角蛋白程序从“过度增殖”到正常模式的正常化都发生了几个月。锚定原纤维的再生也加速了。移植到真皮组织后，朗格汉斯细胞的再增殖和再色素似乎都没有发生显着改变。通过特殊染色，2.25年后仍能看到同种异体真皮的弹性蛋白网络，表明同种移植基质的长期存在。通过临床评估，移植到异种真皮的培养上皮自体移植物的成功移植率（“移植率”）估计约为 95%，并且长期耐久性非常好。
Cultured epithelial autografts transplanted to homograft dermis were analyzed histopathologically from 9 days to 2.25 years after grafting and compared with cultured epithelial autografts transplanted to wound beds devoid of homograft dermis in the same patient. The results suggested that grafting to a dermal substrate accelerates biologic events in skin regeneration from cultured epithelial autografts. Both rete ridge development and normalization of the epidermal keratin program from a "hyperproliferative" to a normal pattern occurred months sooner in the cultured epithelial autografts placed on a dermal matrix. Regeneration of anchoring fibrils was also accelerated. Neither repopulation by Langerhans cells nor repigmentation appeared to be altered significantly by grafting to dermal tissue. By special stain, the elastin network of the allograft dermis could still be visualized at 2.25 years, indicating long-term persistence of the homograft matrix. By clinical assessment the rate of successful engraftment ("take rate") of cultured epithelial autografts grafted to allodermis was estimated to be about 95%, and long-term durability was excellent.