SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis
SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis
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DOI：
10.1101/2024.02.23.581433
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发表时间：
2024-03
期刊：
影响因子：
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通讯作者：
James W. Opzoomer;Rhianna O’Sullivan;J. Sufi;R. Madsen;X. Qin;Ewa Basiarz;C. Tape
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文献类型：
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作者：
James W. Opzoomer;Rhianna O’Sullivan;J. Sufi;R. Madsen;X. Qin;Ewa Basiarz;C. Tape
我们提出了SIGNAL-seq（通过测序的分裂池索引siG-Nalling分析）：一种多重分裂池组合条形码化方法，可同时测量来自3D模型的固定单细胞中的RNA和翻译后修饰（PTM）。SIGNAL-seq PTM测量等同于质谱细胞术，RNA基因检测类似于分池条形码scRNA-seq。通过测量单个细胞中的mRNA配体-受体对和PTM，SIGNAL-seq可以同时揭示肿瘤微环境可塑性的细胞间和细胞内调节。
We present SIGNAL-seq (Split-pool Indexing siG-Nalling AnaLysis by sequencing): a multiplexed splitpool combinatorial barcoding method that simultaneously measures RNA and post-translational modifications (PTMs) in fixed single cells from 3D models. SIGNAL-seq PTM measurements are equivalent to mass cytometry and RNA gene detection is analogous to split-pool barcoding scRNA-seq. By measuring both mRNA ligand-receptor pairs and PTMs in single cells, SIGNAL-seq can simultaneously uncover inter- and intra-cellular regulation of tumour microenvironment plasticity.