Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa.
Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa.
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DOI：
10.1099/ijs.0.63406-0
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发表时间：
2005-05
影响因子：
2.8
通讯作者：
Cheng-Hui Xie;A. Yokota
中科院分区：
文献类型：
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作者：
Cheng-Hui Xie;A. Yokota
用多相方法对从水稻中分离到的5株自由生活的重氮菌进行了特征分析。这些菌株的亲缘关系很近，16S rRNA基因序列的相似性在99-100%之间，DNA-DNA杂交值在70%以上，表明它们代表了一个单一的物种。与其他已知物种相比，它们的16S rRNA和nifH基因序列的相似性分别不超过93%和89%。系统发育距离表明，这些菌株与α-变形杆菌中的其他分类群不同。这些菌株的化学分类特征包括DNA G+C含量(62.1-63.1mol%)，主要的苯二酚体系(Q-10)，主要脂肪酸(18：1 omega7c，clo 19：0 omega8c和16：0)和主要羟基脂肪酸(14：03-OH，18：03-OH和16：03-OH)。根据系统发育和表型分析，这些分离物被认为代表了一个新的属和种，命名为Pleformomonas oryzae gen。Nov.，sp.11月是提议的。模式菌株为F-7(T)(=IAM 15079(T)=ATCC BAA-940(T)=DSM 16300(T))。
Five strains of free-living diazotrophs isolated from rice were characterized by using a polyphasic approach. The strains were found to be very closely related, with 99-100 % 16S rRNA gene sequence similarity and DNA-DNA hybridization values greater than 70 %, suggesting that they represent a single species. When compared with other recognized species, they showed not more than 93 and 89 % similarity for the 16S rRNA and nifH gene sequences, respectively. Phylogenetic distances showed that these isolates were distinct from other taxa within the alpha-Proteobacteria. Chemotaxonomic characteristics of these isolates included the DNA G + C content (62.1-63.1 mol%), the major quinone system (Q-10), predominant fatty acids (18 : 1omega7c, cyclo 19 : 0omega8c and 16 : 0) and major hydroxy fatty acids (14 : 0 3-OH, 18 : 0 3-OH and 16 : 0 3-OH). Based on phylogenetic and phenotypic analyses, these isolates are considered to represent a novel genus and species, for which the name Pleomorphomonas oryzae gen. nov., sp. nov. is proposed. The type strain is F-7(T) (=IAM 15079(T) = ATCC BAA-940(T) = DSM 16300(T)).