Molecular cloning and functional characterization of the zebrafish ATP-gated ionotropic receptor P2X(3) subunit.
Molecular cloning and functional characterization of the zebrafish ATP-gated ionotropic receptor P2X(3) subunit.
复制标题
斑马鱼 ATP 门控离子受体 P2X(3) 亚基的分子克隆和功能表征。
DOI：
10.1016/s0014-5793(00)01685-9
复制
发表时间：
2000
期刊：
影响因子：
3.5
通讯作者：
Voigt,MM
中科院分区：
文献类型：
--
作者：
Egan,TM;Cox,JA;Voigt,MM
我们描述了从斑马鱼 (Danio rerio) 克隆的 P2X 亚基，它是哺乳动物 P2X3 亚基的直系同源物。与哺乳动物 P2X3 一样，该受体在激动剂存在下会迅速脱敏。然而，它的不同之处在于，αβ-meATP 是一种比 ATP 效力低得多的激动剂，并且拮抗剂 TNP-ATP 在低纳摩尔浓度下没有活性。与大鼠 P2X3 亚基类似，斑马鱼亚基与具有不同于亲本表型的大鼠 P2X2 形成异源寡聚体组装体。这种新型克隆将为未来研究 P2X 亚基结构域的结构/功能关系的实验提供重要基础。
We describe a P2X subunit cloned from the zebrafish (Danio rerio) that is an orthologue of the mammalian P2X3subunit. Like the mammalian P2X3, this receptor desensitizes rapidly in the presence of agonist. However, it differs in that αβ-meATP is a much less potent agonist than ATP and the antagonist TNP-ATP is not active at low nanomolar concentrations. Similar to the rat P2X3subunit, the zebrafish subunit forms hetero-oligomeric assemblies with the rat P2X2that possesses a phenotype distinct from either parent. This novel clone will provide an important basis for future experiments investigating the structure/function relationships of P2X subunit domains.