Structural complexity of early embryos:: A study on the nematode Caenorhabditis elegans
Structural complexity of early embryos:: A study on the nematode Caenorhabditis elegans
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DOI：
10.1023/a:1013178514397
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发表时间：
2001-01-01
影响因子：
1.8
通讯作者：
Bignone, FA
中科院分区：
文献类型：
--
作者：
Bignone, FA
为了对基因表达、基因表达控制和细胞相互作用的动力学进行分析研究，线虫秀丽隐杆线虫[C. [1-4]是目前最合适的模型之一。在这种生物体中，遗传图谱和序列是已知的[5]，此外，其谱系树的恒定性允许对产生胚胎的细胞克隆进行完整描述。这些特征促进了对这种生物体发育的几个方面的详细研究。细胞运动的定量研究，通过原肠胚形成的时间推移电影，允许细胞迁移引起的最终胚胎结构的描述。从长远来看，这些研究加上：遗传分析，通过分子技术或其他方法获得的基因表达模式，开辟了在器官尺度上进行动态研究的可能性。这种可能性首先意味着研究空间的划分，并提出了几个问题，以确定在这种研究中使用的基本概念工具。在这方面要处理的主要问题之一是胚胎结构的定量定义。我们将表明，这方面是一个更一般的情况下，距离几何方法，定义在蛋白质折叠的研究。在本文中，我们讨论的措施的复杂性胚胎体计划，在结束的grastrulation。这些可以应用于基因表达动力学的研究和系统发育的研究与进一步的实验或模拟。
For analytical studies on the dynamics of gene expression, gene expression control and cellular interactions, the nematode Caenorhabditis elegans [C. elegans] is at present one of the best suited models [1-4]. In this organism the genetic map and sequence is known [5], moreover the constancy of its lineage tree allows a complete description of cellular clones giving rise to embryos. These characteristics have fostered detailed studies on several aspects of development for this organism. Quantitative studies of cellular movement, through time lapse cinematography of gastrulation, allows the description of cellular migrations giving rise to the final embryonic structure. In perspective, these studies coupled with: genetic analysis, patterns of gene expression obtained through molecular techniques or other methods, open up the possibility of dynamical studies at the organismic scale. This possibility implies, first of all, a study of partitioning of space, and raise several problems in order to define basic conceptual tools to be used in such studies. One of the main problems to handle in this respect is the definition of embryonic structure in a quantitative way. We will show that this aspect is a more general case of distance geometry approaches, as defined in protein folding studies. In this paper we discuss measures of the complexity for embryonal body plans, at the end of grastrulation. These can be applied to studies on the dynamics of gene expression and phylogenetic studies with further experiments or simulations.