A MICROSURGICAL TECHNIQUE FOR RENAL-TRANSPLANTATION IN MICE
A MICROSURGICAL TECHNIQUE FOR RENAL-TRANSPLANTATION IN MICE
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DOI：
10.1111/j.1445-2197.1993.tb00521.x
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发表时间：
1993-03-01
期刊：
影响因子：
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通讯作者：
MOTTRAM, PL
中科院分区：
文献类型：
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作者：
KALINA, SL;MOTTRAM, PL
本文描述了一种用于主要血管化小鼠肾脏移植的改进技术，其中供体是通过暴露左肾，剥离肾上主动脉和腔静脉以及输尿管和膀胱制备的。通过暴露肾下主动脉和腔静脉，并通过近端和远端4/0丝结隔离约1厘米的血管来制备受体。然后将供体肾脏与输尿管和膀胱一起灌注并移至受体。移植手术采用主动脉与主动脉、腔静脉与腔静脉显微外科端侧吻合完成，供体输尿管经膀胱贴片与受体膀胱连接。该技术与先前发表的使用供体肾上主动脉和腔静脉的袖口而不是解剖肾血管底部的技术有显著不同。这些变化使手术更容易，并将缺血时间从35-40分钟减少到25-30分钟，大大提高了器官和受体的存活率。
A modified technique for the transplantation of primarily vascularized mouse kidneys is described in which the donor was prepared by exposure of the left kidney, dissection of the suprarenal aorta and vena cava and of the ureter and bladder. The recipient was prepared by exposure of the infrarenal aorta and vena cava and isolation of approximately 1 cm of these vessels by proximal and distal 4/0 silk ties. The donor kidney was then perfused and removed to the recipient with the ureter and bladder. The transplant was completed by microsurgical end-to-side anastomosis of the aorta to aorta and vena cava to vena cava and the donor ureter was attached to the recipient bladder via a bladder patch. This technique differed significantly from the previously published technique in using cuffs of donor suprarenal aorta and vena cava rather than dissecting the base of the renal vessels. These changes made the operation easier and reduced ischaemia time from 35-40 to 25-30 min, a substantial improvement which increased both organ and recipient survival.