GLUTAMINE TRANSAMINASE-OMEGA-AMIDASE SYSTEM IN RAT AND HUMAN-BRAIN
GLUTAMINE TRANSAMINASE-OMEGA-AMIDASE SYSTEM IN RAT AND HUMAN-BRAIN
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DOI：
10.1111/j.1471-4159.1977.tb10626.x
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发表时间：
1977-01-01
影响因子：
4.7
通讯作者：
GROSS, M
中科院分区：
文献类型：
--
作者：
COOPER, AJL;GROSS, M
来自大鼠脑的谷氨酰胺转氨酶出现在线粒体部分中，而ω-谷氨酰胺转氨酶出现在线粒体部分中。酰胺酶发生在可溶性级分中。这两种酶也广泛分布在人脑组织中。谷氨酰胺转氨酶和ω-谷氨酰胺转氨酶的比活性在死后5小时，1个个体的顶叶皮层中的酰胺酶分别为8和53 μ mol/h/g组织。大鼠脑谷氨酰胺转氨酶与肝线粒体酶相同。谷氨酰胺转氨酶和ω-谷氨酰胺转氨酶的改进测定描述了粗组织匀浆中的酰胺酶活性。谷氨酰胺转氨酶可能的生理重要性及其在肝病脑病中的潜在作用进行了讨论。
Glutamine transaminase from rat brain occurred in the mitochondrial fraction, whereas the .omega.-amidase occurred in the soluble fraction. Both enzymes were also widely distributed throughout human brain tissue. Specific activities for the glutamine transaminase and .omega.-amidase in the parietal cortex of 1 individual at 5 h post mortem were 8 and 53 .mu.mol/h per g of tissue, respectively. Rat brain glutamine transaminase was identical to that of the liver mitochondrial enzyme. Improved assays for glutamine transaminase and .omega.-amidase activities in crude tissue homogenates were described. The possible physiological importance of glutamine transaminase and its potential role in the encephalopathy of hepatic disease are discussed.