Treatment and prevention of rat glioblastoma by immunogenic C6 cells expressing antisense insulin-like growth factor I RNA.
Treatment and prevention of rat glioblastoma by immunogenic C6 cells expressing antisense insulin-like growth factor I RNA.
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通过表达反义胰岛素样生长因子 I RNA 的免疫原性 C6 细胞治疗和预防大鼠胶质母细胞瘤。
DOI：
10.1126/science.8418502
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Ilan,J
中科院分区：
文献类型：
--
作者：
Trojan,J;Johnson,TR;Rudin,SD;Ilan,J;Tykocinski,ML;Ilan,J
大鼠C6胶质瘤细胞表达胰岛素样生长因子I(IGF-I)，并在同基因动物体内形成快速生长的肿瘤。当以Episome为基础的载体编码反义IGF-I互补DNA时，这些细胞失去了致瘤性。皮下注射IGF-I反义C6细胞可预防未转染C6细胞诱发的皮下肿瘤和脑肿瘤的形成。当在肿瘤远端注射时，反义转基因细胞也能引起已建立的脑胶质母细胞瘤的消退。这些抗肿瘤作用是由涉及CD8+淋巴细胞的胶质瘤特异性免疫反应产生的。反义阻断IGF-I的表达可能会逆转C6胶质瘤细胞逃避免疫系统的表型。
Rat C6 glioma cells express insulin-like growth factor I (IGF-I) and form rapidly growing tumors in syngeneic animals. When transfected with an episome-based vector encoding antisense IGF-I complementary DNA, these cells lost tumorigenicity. Subcutaneous injection of IGF-I antisense-transfected C6 cells into rats prevented formation of both subcutaneous tumors and brain tumors induced by nontransfected C6 cells. The antisense-transfected cells also caused regression of established brain glioblastomas when injected at a point distal to the tumor. These antitumor effects result from a glioma-specific immune response involving CD8+lymphocytes. Antisense blocking of IGF-I expression may reverse a phenotype that allows C6 glioma cells to evade the immune system.