Validation and Fine Mapping a Multifunction Region qPCG10/qDC10 Conferring Chalkiness and Grain Shape of Rice
Validation and Fine Mapping a Multifunction Region qPCG10/qDC10 Conferring Chalkiness and Grain Shape of Rice
复制标题
验证和精细映射多功能区域 qPCG10/qDC10 赋予垩白度和颗粒形状
DOI：
10.2135/cropsci2017.07.0405
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发表时间：
2018-03-01
期刊：
影响因子：
2.3
通讯作者：
Wang, Shimei
中科院分区：
文献类型：
--
作者：
Mei, Deyong;Yu, Zhikun;Wang, Shimei
垩白和粒形是稻米的两个重要外观性状，粒形通常被认为对胚乳的垩白有很大影响。本研究旨在验证和精细定位位于水稻第10号染色体长臂上的一个重要多功能区域qPCG 10/qDC 10，该区域赋予粒形和垩白，但尚未得到很好的表征。首先利用籼稻母本特青和轮回父本IR 24的BC 2F 2和BC 2F 2：3群体进行了数量性状座位验证。从BC 4F 2群体中选择在RM 3773至RM 6673区间具有重叠杂合片段的植物以开发用于qPCG 10/qDC 10精细定位的近等基因系。通过对各近等基因系3个基因型组间表型方差的双因素方差分析，将qPCG 10/qDC 10定位在RM 25828和RM 3123之间的93.4kb区域内，对粒宽、粒长、千粒重、垩白粒率和垩白度有显著影响。我们推测该多功能区可能含有一个新的垩白和粒形基因。本研究为qPCG 10/qDC 10基因的克隆和水稻垩白形成的分子机制研究奠定了基础。
Chalkiness and grain shape are two important appearance traits of milled rice (Oryza sativa L.), and grain shape is generally considered to greatly affect chalkiness of endosperm. This study was conducted to validate and fine map an important multifunction region located on the long arm of rice chromosome 10, qPCG10/qDC10, conferring grain shape and chalkiness that has not been well characterized. The quantitative trait loci validations were first performed using BC2F2 and BC2F2:3 populations derived from indica maternal line Teqing and recurrent paternal line IR24. Plants with overlapping heterozygous segments in the interval of RM3773 to RM6673 were selected from the BC4F2 population to develop nearisogenic lines for fine mapping of qPCG10/ qDC10. Using two-way ANOVA of phenotypic variance among three genotype groups in each near-isogenic line set, qPCG10/qDC10 was delimited to a 93.4-kb region flanked by RM25828 and RM3123 and showed significant effects on grain width, grain length, 1000-grain weight, percentage of chalky grain, and degree of chalkiness. We speculated that the multifunction region may harbor a novel gene causing chalkiness and grain shape. The present study will facilitate the cloning of qPCG10/qDC10 and the exploration of the molecular mechanisms underlying rice grain chalkiness.