ANALYSIS OF POLY-BETA-HYDROXYBUTYRATE IN RHIZOBIUM-JAPONICUM BACTEROIDS BY ION-EXCLUSION HIGH-PRESSURE LIQUID-CHROMATOGRAPHY AND UV DETECTION
ANALYSIS OF POLY-BETA-HYDROXYBUTYRATE IN RHIZOBIUM-JAPONICUM BACTEROIDS BY ION-EXCLUSION HIGH-PRESSURE LIQUID-CHROMATOGRAPHY AND UV DETECTION
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DOI：
10.1128/aem.46.6.1339-1344.1983
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
EMERICH, DW
中科院分区：
文献类型：
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作者：
KARR, DB;WATERS, JK;EMERICH, DW
采用离子排斥高压液相色谱法（HPLC）测定了大豆根瘤菌类菌体中聚β-羟基丁酸酯（PHB）的含量。通过HPLC在Aminex HPX-87 H离子排阻树脂上对含PHB材料的酸消化物中的产物进行分级，用于有机酸分析。通过在210 nm处的强吸光度来检测在酸消化过程中由PHB形成的巴豆酸。Aminex-HPLC方法为有机酸的常规分析提供了一种快速、简单的色谱技术。通过气相色谱和分光光度分析证实了通过Aminex-HPLC的PHB分析结果。
Ion-exclusion high-pressure liquid chromatography (HPLC) was used to measure poly-β-hydroxybutyrate (PHB) inRhizobium japonicumbacteroids. The products in the acid digest of PHB-containing material were fractionated by HPLC on Aminex HPX-87H ion-exclusion resin for organic acid analysis. Crotonic acid formed from PHB during acid digestion was detected by its intense absorbance at 210 nm. The Aminex-HPLC method provides a rapid and simple chromatographic technique for routine analysis of organic acids. Results of PHB analysis by Aminex-HPLC were confirmed by gas chromatography and spectrophotometric analysis.