GLUCOCORTICOID RECEPTOR COLOCALIZATION WITH PITUITARY-HORMONES IN THE RAT PITUITARY-GLAND
GLUCOCORTICOID RECEPTOR COLOCALIZATION WITH PITUITARY-HORMONES IN THE RAT PITUITARY-GLAND
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DOI：
10.1016/0303-7207(93)90144-9
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发表时间：
1993-05-01
影响因子：
4.1
通讯作者：
PELTOHUIKKO, M
中科院分区：
文献类型：
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作者：
KONONEN, J;HONKANIEMI, J;PELTOHUIKKO, M
糖皮质激素受体（GR）在垂体前叶的存在以前已被证明，但表达GR的确切细胞类型尚未得到表征。在这项研究中，我们证明了GR和垂体激素的共定位在大鼠脑垂体中使用免疫细胞化学双标记方法。大部分前叶促肾上腺皮质激素免疫反应和生长激素免疫反应细胞含有GR样免疫反应。中间叶细胞呈强ACTH样免疫反应，但不表达GR，糖蛋白激素促甲状腺激素、促卵泡激素和促黄体激素与GR共定位程度较低;对这些激素表现出免疫反应性的细胞中约有一半含有GR。相比之下，只有少数催乳素免疫反应细胞表达GR。我们的结果表明，糖皮质激素可能通过直接影响激素，产生垂体前叶的细胞。
The presence of glucocorticoid receptor (GR) in the anterior lobe of the pituitary gland has previously been demonstrated, but the exact cell types expressing GR have not yet been characterized. In this study, we demonstrate the colocalization of GR and pituitary hormones in the rat pituitary gland by using an immunocytochemical double-labelling method. The majority of anterior lobe corticotropin-immunoreactive and growth hormone-immunoreactive cells contained GR-like immunoreactivity. Cells of the intermediate lobe showed intensive ACTH-like immunoreactivity but did not express GR. The glycoprotein hormones thyroid-stimulating hormone, follicle-stimulating hormone and luteinizing hormone were colocalized with GR to a lesser degree; approximately one-half of the cells exhibiting immunoreactivity to these hormones contained GR. By contrast, only a minority of the prolactin-immunoreactive cells expressed GR. Our results suggest that glucocorticoids may differentially regulate the secretion and/or synthesis of these pituitary hormones by directly affecting the hormone-producing cells of the anterior pituitary.