Field desorption mass spectrometry using needleless emitters. A study of five synthetic tripeptides as hormone models
Field desorption mass spectrometry using needleless emitters. A study of five synthetic tripeptides as hormone models
复制标题
使用无针发射器进行场解吸质谱分析。
DOI：
10.1021/ja00487a045
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发表时间：
1978
影响因子：
15
通讯作者：
K. Folkers
中科院分区：
文献类型：
--
作者：
W. Frick;E. Barofsky;G. Daves;D. Barofsky;D. Chang;K. Folkers
结果记录光谱。研究的肽的典型场解吸质谱如图1和2所示。由于发射器上的样品量很小，因此用于记录的足够强的离子电流只能维持很短的时间。记录与解吸曲线的同步允许在样品解吸的特定阶段记录光谱（参见图la和lb）。一旦在特定的发射器温度下开始，整个样品的解吸通常发生在比扫描整个质量范围所需的时间间隔更短的时间间隔内。对于扩展的质量范围，发现有必要在连续实验中记录选定区域，以获得足够的低丰度离子强度，从而进行准确的质量分配（见图2）。由于这些实验的情况下，间-
ResultsRecording of Spectra. Typical field desorption mass spectra of the peptides studied are shown in Figures 1 and 2. Due to the small amount of sample on the emitter, a sufficiently in-tense ion current for recording can be maintained only for a short period of time. Synchronization of the recording with the desorption profile allows the recording of spectra at a particular stage of sample desorption (see Figures la and lb). Desorption of an entire sample, once initiated at a specific emitter temperature, usually occurs in a time interval shorter than that required to scan the entire mass range. For extended mass ranges, it was found necessary to record selected regions in sequential experiments in order to obtain sufficient inten-sities of low abundance ions for accurate mass assignment (see Figure 2). Dueto these experimental circumstances, inter-