Solution NMR studies of integral polytopic a-helical membrane proteins: the structure determination of the seven-helix transmembrane receptor sensory rhodopsin II, pSRII.
Solution NMR studies of integral polytopic a-helical membrane proteins: the structure determination of the seven-helix transmembrane receptor sensory rhodopsin II, pSRII.
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整体多面α螺旋膜蛋白的溶液核磁共振研究：七螺旋跨膜受体感觉视紫红质II，pSRII的结构测定。
DOI：
10.1007/978-1-62703-023-6_3
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发表时间：
2012
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影响因子：
--
通讯作者：
Gautier A
中科院分区：
文献类型：
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作者：
Gautier A
基因组中编码的蛋白质中约有30%以膜蛋白的形式表达，但这些蛋白质仅占今天已解决的所有结构的不到1%。鉴于膜蛋白的生理和药物意义，很明显，需要更好和更全面地了解它们在原子分辨率下的三维（3D）结构。α-螺旋整合膜蛋白通常比β-桶型蛋白更难处理，这对于多位成员如七螺旋蛋白大家族尤其如此。在这一章中，我们描述了实际方面的溶液状态的NMR光谱结构测定的七螺旋跨膜（7-TM）蛋白受体感觉视紫红质pSRII从haloalkaliphilic archaeonNatronomonaspharaonisreconstituted在洗涤剂胶束。这是首次通过NMR确定7-TM蛋白质的三维结构。
About 30% of the proteins encoded in the genome are expressed as membrane proteins but these represent <1% of all the structures solved today. In view of the physiological and pharmaceutical significance of membrane proteins it is clear that a better and more comprehensive understanding of their three-dimensional (3D) structures at atomic resolution is required. α-Helical integral membrane proteins are generally more difficult to work with than β-barrel-type proteins and this has particularly been true for the polytopic members such as the large family of seven-helical proteins. In this chapter we describe the practical aspects of the solution-state NMR spectroscopy structure determination of the seven-helical transmembrane (7-TM) protein receptor sensory rhodopsin pSRII from the haloalkaliphilic archaeonNatronomonas pharaonisreconstituted in detergent micelles. This is the first time that a three-dimensional structure of a 7-TM protein has been determined by NMR.