Regulation of fibrinolytic protein adsorption on polyurethane surfaces by modification with lysine-containing copolymers
Regulation of fibrinolytic protein adsorption on polyurethane surfaces by modification with lysine-containing copolymers
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DOI：
10.1039/c3py00710c
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发表时间：
2013-10
影响因子：
4.6
通讯作者：
Zengchao Tang;Xiaoli Liu;Yafei Luan;Wei Liu;Zhaoqiang Wu;Dan Li;Hong Chen
中科院分区：
文献类型：
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作者：
Zengchao Tang;Xiaoli Liu;Yafei Luan;Wei Liu;Zhaoqiang Wu;Dan Li;Hong Chen
报道了一种调节纤溶酶原及其激活剂t-PA附着到聚氨酯表面的新策略。该方法基于含有赖氨酸的甲基丙烯酸羟乙酯(HEMA)的一步接枝共聚。共聚物改性的PU表面具有较高的纤溶酶原结合能力和t-PA结合能力。还研究了预吸附的t-PA从含赖氨酸的PU表面与等离子体接触时的释放。结果表明，通过改变单体投料比，从而改变接枝物中HEMA和赖氨酸的相对含量，可以简单地控制纤溶酶原与t-PA在这类表面上的相互作用。这种方法可能有助于开发纤溶、血液接触材料，更广泛地说，用于控制蛋白质与表面的相互作用以实现生物相容性。
A new strategy to regulate the attachment of plasminogen and its activator, t-PA, to a polyurethane surface is reported. The method is based on the one-step graft copolymerization of a lysine-containing methacrylic monomer and hydroxyethyl methacrylate (HEMA). The copolymer-modified PU surfaces showed high capacity for plasminogen and t-PA binding. The release of pre-adsorbed t-PA from the lysine-containing PU surfaces in contact with plasma was also investigated. It was shown that the interactions of plasminogen and t-PA on this type of surface can be controlled simply by changing the monomer feed ratio, and thus the relative quantities of HEMA and lysine in the grafts. This approach may be useful for the development of fibrinolytic, blood-contacting materials and, more generally, for controlling protein-surface interactions for biocompatibility.