Targeted Analysis of Conjugated and Microbial-Derived Phenolic Metabolites in Human Urine After Consumption of an Almond Skin Phenolic Extract
Targeted Analysis of Conjugated and Microbial-Derived Phenolic Metabolites in Human Urine After Consumption of an Almond Skin Phenolic Extract
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DOI：
10.3945/jn.110.124065
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发表时间：
2010-10-01
影响因子：
4.2
通讯作者：
Andres-Lacueva, Cristina
中科院分区：
文献类型：
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作者：
Garrido, Ignacio;Urpi-Sarda, Mireia;Andres-Lacueva, Cristina
与16名健康志愿者（7名男性和5名女性）进行了一项单盲，安慰剂对照和随机试验研究。测试组摄入了包含杏仁的皮肤酚类提取物（884毫克的总多酚/剂量），其中含有黄酮-3-醇，黄酮醇和黄酮，而安慰剂组摄入了微晶纤维素。我们在这项研究中的目的是确定在（-2至0 h）之前（-2至0 h）和之后（0-2、2-6、6-10和10-24 h）杏仁多酚组的尿液排泄的变化。对于测试组，（EPI）儿茶素和Naringenin结合物的最大尿液排泄在消耗杏仁皮肤提取物和排泄后2-6小时与安慰剂组在此期间在2-6小时后获得（p 0.05）。消耗杏仁多酚后6-10小时，羟基苯基伏甲酰胺乳元达到最大尿液水平，在这段时间内排泄与安慰剂组不同（p = 0.0004）。对于测试组，酚酸的排泄（羟基苯基丙酸，羟基苯基乙酸，羟基苯甲酸和羟基霉素酸）在任何时间尿液收集时间（p> 0.05）在任何时间段（p> 0.05）与安慰剂组没有差异。这项工作中提出的发现提供了有关考虑II期和微生物代谢的影响的杏仁皮肤多酚的生物利用度的证据。 J. Nutr。 140：1799-1807，2010。
A single-blind, placebo-controlled, and randomized trial study was carried out with 16 healthy volunteers (7 men and 5 women). The test group ingested an encapsulated almond skin phenolic extract (884 mg of total polyphenols/dose) containing flavan-3-ols, flavonols, and flavanones, whereas the placebo group ingested microcrystalline cellulose. Our aim in this study was to determine changes in the urinary excretion of conjugated and microbial-derived phenolic metabolites before (-2 to 0 h) and after (0-2, 2-6, 6-10, and 10-24 h) intake of the almond polyphenols compared with the placebo group. For the test group, maximum urinary excretion of (epi)catechin and naringenin conjugates derived from phase II metabolism was attained at 2-6 h after consumption of the almond skin extract and excretions differed from the placebo group during this time period (P 0.05). Hydroxyphenylvalerolactones reached maximum urinary levels at 6-10 h after consumption of almond polyphenols, and excretion differed from the placebo group during this time period (P = 0.0004). For the test group, excretions of phenolic acids (hydroxyphenylpropionic, hydroxyphenylacetic, hydroxybenzoic, and hydroxycinnamic acids) did not differ from the placebo group at any time period of urine collection (P > 0.05). The findings presented in this work provide evidence concerning the bioavailability of almond skin polyphenols considering the effects of both phase II and microbial metabolism. J. Nutr. 140: 1799-1807, 2010.