Monitoring bacterial contamination in equine platelet concentrates obtained by the tube method in a clean laboratory environment under three different technical conditions
Monitoring bacterial contamination in equine platelet concentrates obtained by the tube method in a clean laboratory environment under three different technical conditions
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DOI：
10.2746/042516409x455221
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发表时间：
2010-01-01
影响因子：
2.2
通讯作者：
Carmona, J. U.
中科院分区：
文献类型：
--
作者：
Alvarez, M. E.;Giraldo, C. E.;Carmona, J. U.
开展研究的原因：人们对使用自体血小板浓缩物（PCs）治疗马的慢性肌肉骨骼疾病越来越感兴趣。然而，在制备过程中没有关于细菌污染风险的信息。目的：1)评估在3种技术条件下，通过管法获得的马pc的细菌污染风险：a)在层流柜中，在清洁的实验室环境中，有(b)和没有(c)本生灯；2)识别pc制备过程中存在细菌污染风险的关键点；3)识别过程中潜在的细菌污染物及其抗生素敏感性。方法：对15匹马进行消毒前后的静脉穿刺部位皮肤（剃毛或未剃毛）进行细菌学检测；操作员的手和喉咙；处理血液的管子的盖子；采集马血样的环境；实验室环境;层流柜；细菌学的火炉;以及在3种技术条件下获得的pc。结果：无菌制备的马皮肤、操作者的手和咽喉以及采血处均分离出细菌。管盖、实验室环境、层流柜、pc等均未分离出细菌。分离的细菌为马皮肤、人皮肤和喉部的正常菌群，以及环境污染物。其中，对青霉素耐药的占23%，对氨苄西林耐药的占19%，对头孢噻呋耐药的占2.12%，对磺胺甲恶唑/甲氧苄啶耐药的占3.2%，对恩诺沙星耐药的占1.1%。未见对阿米卡星和庆大霉素耐药。结论和潜在的相关性：在干净的实验室环境中，不需要层流柜或本生灯，可以通过管法获得未污染的pc。必须按照严格的无菌技术进行操作。
Reasons for performing study: There is a growing interest in the use of autologous platelet concentrates (PCs) as treatment for chronic musculoskeletal diseases in horses. However, there is no information on the risk of bacterial contamination during their preparation.Objectives: To: 1) assess the risk of bacterial contamination in equine PCs obtained by the tube method under 3 technical conditions: a) in a laminar flow cabinet, in a clean laboratory environment both with (b) and without (c) Bunsen burner; 2) identify the critical points of the process of PCs preparation with risk of bacterial contamination; and 3) identify the potential bacterial contaminants in the process and their antibiotic susceptibility.Methods: Bacteriological samples were taken from: the skin (shaved or unshaved) of the venipuncture site in 15 horses, both before and after being disinfected; hands and throat of the operator; caps of the tubes where the blood was processed; environment where the equine blood samples were collected; laboratory environment; laminar flow cabinet; bacteriological stove; and PCs obtained under 3 technical conditions.Results: Bacteria were isolated from nonaseptically prepared equine skin, hands and throat of the operator, and the place where the blood samples were taken. Bacteria were not isolated from tube caps, laboratory environment, laminar flow cabinet or PCs. The isolated bacteria were normal biota from equine skin, human skin and throat, and environmental contaminants. Of the isolated bacteria, 23% were resistant to penicillin, 19% to ampicillin, 2.12% to ceftiofur, 3.2% to sulphamethoxazole/trimethoprim and 1.1% to enrofloxacin. Resistance to amikacin and gentamicin was not seen.Conclusions and potential relevance: Uncontaminated PCs can be obtained by the tube method in a clean laboratory environment without the need for either a laminar flow cabinet or a Bunsen burner. It is mandatory to perform the procedure following strict aseptic technique.