Functional correlates of the interleukin-1 receptor antagonist gene polymorphism in the colonic mucosa in ulcerative colitis
Functional correlates of the interleukin-1 receptor antagonist gene polymorphism in the colonic mucosa in ulcerative colitis
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DOI：
10.1038/sj.gene.6364032
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发表时间：
2004-01-01
影响因子：
5
通讯作者：
di Giovine, FS
中科院分区：
文献类型：
--
作者：
Carter, MJ;Jones, S;di Giovine, FS
相关研究已经确定白细胞介素-1受体拮抗剂基因等位基因2（IL-1RN*2）是溃疡性结肠炎（UC）的易感性标志。本研究探讨IL-1RN基因型对结肠黏膜蛋白和mRNA表达的影响。使用ELISA检测99例UC患者和54例对照组直肠活检匀浆中细胞因子IL-1ra、IL-1a和IL-1b的含量。采用限制性内切酶法测定IL1RN、IL1A和IL1B基因型。在7例杂合患者的结肠黏膜中评估了两个IL1RN等位基因产生稳态mRNA积累的能力。逐步线性回归结果显示，IL-1RN基因型（P=0.001）、诊断（P=0.001）
Association studies have identified the interleukin-1 receptor antagonist gene allele 2(IL-1RN*2) as a marker of susceptibility in ulcerative colitis (UC). This study investigated the significance of the IL-1RN genotype with respect to protein and mRNA expression in the colonic mucosa. Homogenates of rectal biopsies from 99 UC and 54 controls were assayed for cytokines IL-1ra, IL-1a and IL-1b using ELISA. IL1RN, IL1A and IL1B genotypes were determined using restriction-enzyme analysis. The ability of the two IL1RN alleles to generate steady-state mRNA accumulation was assessed in the colonic mucosa of seven heterozygous patients. Stepwise linear regression demonstrated that IL-1RN genotype (P=0.001), diagnosis (P