Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells
Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells
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DOI：
10.1016/j.bbrc.2010.04.116
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发表时间：
2010-05-28
影响因子：
3.1
通讯作者：
O'Driscoll, Lorraine
中科院分区：
文献类型：
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作者：
Hennessy, Erica;Clynes, Martin;O'Driscoll, Lorraine
MicroRNA (miRNA) 是一类内源性小非编码 RNA，可在转录后水平调节 mRNA。 miRNA 在正常生理和病理条件下均已被鉴定。迄今为止，有限数量的 miRNA 已被证明参与胰岛素分泌的调节。使用 TaqMan 低密度 miRNA 阵列，我们鉴定了一组 10 个 miRNA 在葡萄糖非反应性 MIN6 细胞中比葡萄糖反应性细胞下调。在这 10 个 miRNA 靶标中，随后涉及 mir-200a、mir-130a 和 mir-410 水平敲低的功能研究表明，它们可能会降低 MIN6 细胞响应葡萄糖刺激水平分泌胰岛素的能力。相反，mir-410 过度表达的实验表明，它可能会增强葡萄糖刺激的胰岛素分泌水平。在这项研究中，我们还鉴定了 21 种以前未知的 miRNA，它们具有潜在的鼠类同源物。 (C) 2010 Elsevier Inc. 保留所有权利。
MicroRNAs (miRNAs) are a family of endogenous small non-coding RNAs which regulate mRNAs at the post-transcriptional level. MiRNAs have been identified in both normal physiological and pathological conditions. To date, a limited number of miRNAs have been shown to be involved in the regulation of insulin secretion. We have identified a panel of 10 miRNAs down-regulated in glucose non-responsive MIN6 cells compared to glucose responsive cells using TaqMan Low Density miRNA Arrays. Of these 10 miRNA targets, subsequent functional investigations involving knockdown of mir-200a, mir-130a and mir-410 levels suggested that they may decrease the capability of MIN6 cells to secrete insulin in response to stimulatory levels of glucose. Conversely, experiment with over-expression of mir-410 suggest that it may enhance levels of glucose stimulated insulin secretion. In this study, we have also identified 21 miRNAs not previously known to have a potential murine homologue. (C) 2010 Elsevier Inc. All rights reserved.