ARD1 AND NAT1 PROTEINS FORM A COMPLEX THAT HAS N-TERMINAL ACETYLTRANSFERASE ACTIVITY
ARD1 AND NAT1 PROTEINS FORM A COMPLEX THAT HAS N-TERMINAL ACETYLTRANSFERASE ACTIVITY
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DOI：
10.1002/j.1460-2075.1992.tb05267.x
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发表时间：
1992-06-01
期刊：
影响因子：
11.4
通讯作者：
SZOSTAK, JW
中科院分区：
文献类型：
--
作者：
PARK, EC;SZOSTAK, JW
两个酵母基因，ARD 1和NAT 1，需要表达的N-末端蛋白乙酰转移酶。这种活性是沉默交配型位点HML的完全抑制、孢子形成和进入G 0所必需的。虽然NAT 1基因产物被认为是该酶的催化亚基，但ARD 1蛋白的作用仍不清楚。我们已经使用表位标记的衍生物的ARD 1和NAT 1的ARD 1-NAT 1复合物的形成提供了生化证据，并显示，这两种蛋白质所需的N-末端乙酰转移酶活性。我们还提出了ARD 1-ARD 1同源二聚体形成的证据。缺失分析表明，ARD 1的C-末端区域可能参与了ARD 1-ARD 1和ARD 1-NAT 1复合物的形成。
Two yeast genes, ARD1 and NAT1, are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating type locus HML, for sporulation, and for entry into G0. While the NAT1 gene product is thought to be the catalytic subunit of the enzyme, the role of the ARD1 protein has remained unclear. We have used epitope tagged derivatives of ARD1 and NAT1 to provide biochemical evidence for the formation of an ARD1-NAT1 complex, and to show that both proteins are required for the N-terminal acetyltransferase activity. We also present evidence for the formation of ARD1-ARD1 homodimers. Deletion analysis suggests that the C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes.