CHARACTERIZATION OF A HIGH MOLECULAR-WEIGHT PROTECTIVE ANTIGEN OF PLASMODIUM-YOELII
CHARACTERIZATION OF A HIGH MOLECULAR-WEIGHT PROTECTIVE ANTIGEN OF PLASMODIUM-YOELII
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DOI：
10.1017/s0031182000054482
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发表时间：
1984-01-01
期刊：
影响因子：
2.4
通讯作者：
FREEMAN, RR
中科院分区：
文献类型：
--
作者：
HOLDER, AA;FREEMAN, RR
表征了230，000 MW约氏疟原虫保护性抗原。抗约氏疟原虫的两种单克隆抗体免疫沉淀230，000MW蛋白质和许多较低MW多肽。这些多肽通过肽图谱和特异性抗体结合显示为大蛋白的片段。碘化实验表明，低分子量的物种可能存在于裂殖子的表面上。蛋白质未被糖基化。通过血清学检测，相关抗原与长春疫霉的血液阶段感染有关，夏氏黑腐病菌和恶性疟原虫。
A 230,000 MW P. yoelii protective antigen was characterized. Two monoclonal antibodies against P. yoelii immunoprecipitated the 230,000 MW protein and a number of lower MW polypeptides. These polypeptides were shown by peptide mapping and specific antibody binding to be fragments of the large protein. Iodination experiments suggested that the lower MW species may be present on the surface of the merozoite. The protein was not glycosylated. By serology, related antigens were associated with blood-stage schizonts of P. vinckei sspp., P. chabaudi sspp. and P. falciparum.