Improved Humoral Immunity and Protection against Influenza Virus Infection with a 3d Porous Biomaterial Vaccine.
Improved Humoral Immunity and Protection against Influenza Virus Infection with a 3d Porous Biomaterial Vaccine.
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用3d多孔生物材料疫苗提高体液免疫和对流感病毒感染的保护。
DOI：
10.1002/advs.202302248
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发表时间：
2023-11
期刊：
影响因子：
15.1
通讯作者：
Di Carlo, Dino
中科院分区：
文献类型：
--
作者：
Miwa, Hiromi;Antao, Olivia Q.;Kelly-Scumpia, Kindra M.;Baghdasarian, Sevana;Mayer, Daniel P.;Shang, Lily;Sanchez, Gina M.;Archang, Maani M.;Scumpia, Philip O.;Weinstein, Jason S.;Di Carlo, Dino
New vaccine platforms that activate humoral immunity and generate neutralizing antibodies are required to combat emerging pathogens, including influenza virus. A slurry of antigen‐loaded hydrogel microparticles that anneal to form a porous scaffold with high surface area for antigen uptake by infiltrating immune cells as the biomaterial degrades is demonstrated to enhance humoral immunity. Antigen‐loaded‐microgels elicited a robust cellular humoral immune response, with increased CD4+ T follicular helper (Tfh) cells and prolonged germinal center (GC) B cells comparable to the commonly used adjuvant, aluminum hydroxide (Alum). Increasing the weight fraction of polymer material led to increased material stiffness and antigen‐specific antibody titers superior to Alum. Vaccinating mice with inactivated influenza virus loaded into this more highly cross‐linked formulation elicited a strong antibody response and provided protection against a high dose viral challenge. By tuning physical and chemical properties, adjuvanticity can be enhanced leading to humoral immunity and protection against a pathogen, leveraging two different types of antigenic material: individual protein antigen and inactivated virus. The flexibility of the platform may enable design of new vaccines to enhance innate and adaptive immune cell programming to generate and tune high affinity antibodies, a promising approach to generate long‐lasting immunity. Tuning physical and chemical properties of a particle‐based hydrogel scaffold enhanced adjuvanticity and promoted humoral immunity and protection against a pathogen when using individual protein antigen and inactivated virus. The modularity of the synthetic material adjuvant can enable design of new vaccines to enhance innate and adaptive immune cell programming to generate and tune high affinity antibodies for long‐lasting immunity.
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影响因子：
41.2
作者：
Griffin DR;Archang MM;Kuan CH;Weaver WM;Weinstein JS;Feng AC;Ruccia A;Sideris E;Ragkousis V;Koh J;Plikus MV;Di Carlo D;Segura T;Scumpia PO
通讯作者：
Scumpia PO
影响因子：
4.4
作者：
Li, Hanfen;Willingham, Stephen B.;Ting, Jenny P. -Y.;Re, Fabio
通讯作者：
Re, Fabio
影响因子：
32.4
作者：
Choi YS;Kageyama R;Eto D;Escobar TC;Johnston RJ;Monticelli L;Lao C;Crotty S
通讯作者：
Crotty S
影响因子：
24.8
作者：
Krishnaswamy JK;Gowthaman U;Zhang B;Mattsson J;Szeponik L;Liu D;Wu R;White T;Calabro S;Xu L;Collet MA;Yurieva M;Alsén S;Fogelstrand P;Walter A;Heath WR;Mueller SN;Yrlid U;Williams A;Eisenbarth SC
通讯作者：
Eisenbarth SC
影响因子：
5.4
作者：
Franchi, Luigi;Nunez, Gabriel
通讯作者：
Nunez, Gabriel