Production of a desirable Brassica oleraceaCMS line using an alloplasmic B. rapaCMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant
Production of a desirable Brassica oleraceaCMS line using an alloplasmic B. rapaCMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant
复制标题
使用携带 Diplotaxis erucoides 细胞质的异质 B. rapaCMS 系作为桥梁植物生产理想的甘蓝 CMS 系
DOI：
10.1111/pbr.12565
复制
发表时间：
2018
期刊：
影响因子：
2
通讯作者：
Sang Woo Bang
中科院分区：
文献类型：
--
作者：
Yoshiaki Fujita; Keita Sunaga; Su‐Hyeun Shim; Wataru Yamada; Takayuki Ohnishi; Sang Woo Bang
甘蓝杂种F1的制种主要利用改良的甘蓝细胞质雄性不育系(CMS)。然而，依赖某一特定品系是有风险的，开发其他CMS品系将是有利的。在本研究中，为了避免供体和受体植株之间的不亲和性，我们利用异源胞质B.rapaCMS作为桥梁植物，将二倍体芥菜细胞质转移到大白菜上。新育大麦不育系由4个世代回交获得，花药不发育，花药小，无花粉粒，表现完全雄性不育。其他花器官没有功能缺陷，接受正常花粉的能力似乎也没有受到损害。此外，携带甘蓝细胞质的甘蓝不育系叶面积较大，胞质正常。因此，携带甘蓝胞质的甘蓝不育系有可能成为商业甘蓝杂交制种的有价值的替代品。
InBrassica oleracea, production of F1hybrid seeds mainly makes use of the improvedOguracytoplasmic male sterile (CMS) line. However, reliance on one particular line is a risk, and it would be advantageous to develop other CMS lines. In this study, we transferredDiplotaxis erucoidescytoplasm toB. oleraceacultivars using an alloplasmicB. rapaCMS line as a bridge plant to avoid incompatibility between donor and recipient plants. The newB. oleraceaCMS lines, which were derived by four generations of backcrossing, had small rudimentary anthers with no pollen grain and showed complete male sterility. There was no functional defect in other floral organs, and the ability to receive normal pollen did not appear to be impaired. Moreover, theB. oleraceaCMS lines carryingD. erucoidescytoplasm had larger leaf areas and a normal plastochron. As a consequence, theB. oleraceaCMS lines carryingD. erucoidescytoplasm have the potential to be valuable alternatives for use in commercialB. oleraceahybrid seed production.