Functional mapping in pea, as an aid to the candidate gene selection and for investigating synteny with the model legume Medicago truncatula
Functional mapping in pea, as an aid to the candidate gene selection and for investigating synteny with the model legume Medicago truncatula
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DOI：
10.1007/s00122-005-0205-y
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发表时间：
2006-04-01
影响因子：
5.4
通讯作者：
Burstin, J
中科院分区：
文献类型：
--
作者：
Aubert, G;Morin, J;Burstin, J
鉴定引起表型性状数量和质量变异的分子多态性是当前农学研究的主要目标。定位克隆、转座子标记以及候选基因策略等多种方法已被用于发现植物中这种变异背后的基因。功能图谱的构建，即由已知功能的基因组成，是候选基因方法的重要组成部分。在本文中，我们报告的63个单核苷酸多态性标记和15个单链构象多态性标记的主要参与初级代谢的酶编码基因的发展，并利用两个豌豆重组自交系群体的复合图谱上的遗传定位。完整的遗传图谱覆盖1，458 cM，包括363个位点，包括总共111个基因锚定标记：本研究中描述的77个基因锚定标记，位于基因序列中的7个微卫星，16个开花时间基因，Tri基因，5个形态标记和5个其他基因。相邻标记之间的平均间距为4 cM，90%的标记与相邻标记的距离小于10 cM。我们还报道了其中21个基因在蒺藜苜蓿中的遗传定位，并在豌豆和蒺藜苜蓿之间增加了41个新的连锁。truncatula地图。我们讨论了使用这种新的复合功能图为未来的候选基因的方法在豌豆。
The identification of the molecular polymorphisms giving rise to phenotypic trait variability-both quantitative and qualitative-is a major goal of the present agronomic research. Various approaches such as positional cloning or transposon tagging, as well as the candidate gene strategy have been used to discover the genes underlying this variation in plants. The construction of functional maps, i.e. composed of genes of known function, is an important component of the candidate gene approach. In the present paper we report the development of 63 single nucleotide polymorphism markers and 15 single-stranded conformation polymorphism markers for genes encoding enzymes mainly involved in primary metabolism, and their genetic mapping on a composite map using two pea recombinant inbred line populations. The complete genetic map covers 1,458 cM and comprises 363 loci, including a total of 111 gene-anchored markers: 77 gene-anchored markers described in this study, 7 microsatellites located in gene sequences, 16 flowering time genes, the Tri gene, 5 morphological markers, and 5 other genes. The mean spacing between adjacent markers is 4 cM and 90% of the markers are closer than 10 cM to their neighbours. We also report the genetic mapping of 21 of these genes in Medicago truncatula and add 41 new links between the pea and M. truncatula maps. We discuss the use of this new composite functional map for future candidate gene approaches in pea.